A rabbit model for capsular contracture: Development and clinical implications
A rabbit model for capsular contracture: Development and clinical implications
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DOI：
10.1097/01.prs.0000208306.79104.18
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发表时间：
2006-04-01
影响因子：
3.6
通讯作者：
Brown, SA
中科院分区：
文献类型：
--
作者：
Adams, WP;Haydon, AS;Brown, SA
背景：包膜挛缩仍然是乳房美容和重建手术中最常见的并发症之一；然而，其原因，预防和治疗仍有待充分阐明。目前，还没有准确的、可重复的体外或体内病理模型来检测包膜挛缩。本研究的目的是建立一个有效的病理包膜挛缩动物模型，模拟人类包膜挛缩反应的形成。方法：将32只新西兰大白兔再分为实验组（n = 16）和对照组（n = 16）。每个亚组在背部区域的肉环下放置光滑盐水微型植入物（30cc）。此外，实验组将纤维蛋白胶作为诱导囊挛缩剂注入种植体口袋。术后2 ~ 8周对家兔实施安乐死。在动物被安乐死之前，每个植入物都连续用生理盐水充气，并使用Stryker装置绘制压力-体积曲线来评估挛缩程度。收集有代表性的胶囊样品并进行组织学检查。正常和收缩的人体包膜组织样本也从接受乳房植入翻修和更换手术的患者中收集。对组织样本进行组织学评估。结果：压力-容积曲线显示实验组囊内压力明显高于对照组。实验组的胶囊比对照组更厚，透明度更低。兔胶囊的组织学评价与对照组和实验亚组的人胶囊相似。结论：家兔病理性包膜挛缩是可靠的。该动物模型为未来研究测试各种全身或局部药物对减轻包膜挛缩的影响提供了框架。
Background: Capsular contracture remains one of the most common complications involving aesthetic and reconstructive breast surgery; however, its cause, prevention, and treatment remain to be fully elucidated. Presently, there is no accurate and reproducible pathologic in vitro or in vivo model examining capsular contracture. The purpose of this study was to establish an effective pathologic capsular contracture animal model that mimics the formation of capsular contracture response in humans.Methods: New Zealand White rabbits (n = 32) were Subdivided into experimental (n = 16) and control groups (n = 16). Each subgroup underwent placement of smooth saline mini implants (30 cc) beneath the panniculus carnosus in the dorsal region of the back. In addition, the experimental group underwent instillation of fibrin glue into the implant pocket as a capsular contracture-inducing agent. Rabbits were euthanized from 2 to 8 weeks after the procedure. Before the animals were euthanized, each implant was serially inflated with saline and a pressure-volume curve was developed using a Stryker device to assess the degree of contracture. Representative capsule samples were collected and histologically examined. Normal and contracted human capsular tissue samples were also collected from patients undergoing breast implant revision and replacement procedures. Tissue samples were assessed histologically.Results: Pressure-volume curves demonstrated a statistically significantly increased intracapsular pressure in the experimental group compared with the control group. The experimental subgroup had thicker, less transparent capsules than the control group. Histologic evaluation of the rabbit capsule was similar to that of the human capsule for the control and experimental subgroups.Conclusions: The authors conclude that pathologic capsular contracture can be reliably induced in the rabbit. This animal model provides the framework for future investigations testing the effects of various systemic or local agent's on reduction of capsular contracture.