Side population cells isolated from KATO III human gastric cancer cell line have cancer stem cell-like characteristics.
Side population cells isolated from KATO III human gastric cancer cell line have cancer stem cell-like characteristics.
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DOI：
10.3748/wjg.v18.i33.4610
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发表时间：
2012-09
影响因子：
4.3
通讯作者：
J. She;Peng-Ge Zhang;Xuan Wang;X. Che;Zi-Ming Wang
中科院分区：
文献类型：
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作者：
J. She;Peng-Ge Zhang;Xuan Wang;X. Che;Zi-Ming Wang
目的探讨侧群(SP)细胞在体外是否具有肿瘤干细胞样特性以及SP细胞在胃癌致瘤过程中的作用。方法我们分析了不同人胃癌细胞系中SP细胞的存在，然后通过流式细胞术从KATO III人胃癌细胞系中分离和鉴定SP细胞。通过克隆和球体形成测定评估克隆形成能力和自我更新。通过逆转录聚合酶链反应确定相关基因。为了比较致瘤能力，将来自 KATO III 人胃癌细胞系的 SP 和非侧群 (NSP) 细胞皮下注射到裸鼠体内。结果 KATO III 中 SP 细胞占总细胞群的 0.57%，Hs-746T 中占 1.04%，AGS (CRL-1739) 中占 0.02%。 SP细胞在条件培养基中可以克隆生长并具有自我更新能力。 SP和NSP细胞之间ABCG2、MDRI、Bmi-1和Oct-4的表达存在差异。然而，SP和NSP细胞注射到裸鼠体内后没有明显差异。结论 SP细胞在体外具有一定的肿瘤干细胞样特征，可用于研究胃癌的致瘤过程。
AIM To investigate whether the side population (SP) cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer. METHODS We analyzed the presence of SP cells in different human gastric carcinoma cell lines, and then isolated and identified the SP cells from the KATO III human gastric cancer cell line by flow cytometry. The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays. The related genes were determined by reverse transcription polymerase chain reaction. To compare tumorigenic ability, SP and non-side population (NSP) cells from the KATO III human gastric cancer cell line were subcutaneously injected into nude mice. RESULTS SP cells from the total population accounted for 0.57% in KATO III, 1.04% in Hs-746T, and 0.02% in AGS (CRL-1739). SP cells could grow clonally and have self-renewal capability in conditioned media. The expression of ABCG2, MDRI, Bmi-1 and Oct-4 was different between SP and NSP cells. However, there was no apparent difference between SP and NSP cells when they were injected into nude mice. CONCLUSION SP cells have some cancer stem cell-like characteristics in vitro and can be used for studying the tumorigenic process in gastric cancer.