Control of magnocellular oxytocin neurones by the region anterior and ventral to the third ventricle (AV3V region) in rats.
Control of magnocellular oxytocin neurones by the region anterior and ventral to the third ventricle (AV3V region) in rats.
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大鼠第三脑室前部和腹侧区域（AV3V 区域）对大细胞催产素神经元的控制。
DOI：
10.1677/joe.0.1140253
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发表时间：
1987
影响因子：
4
通讯作者：
J. Russell
中科院分区：
文献类型：
--
作者：
R. E. Blackburn;G. Leng;J. Russell
利用电刺激和电解损毁技术研究了大鼠第三脑室前腹侧区（AV 3V区）参与催产素释放的控制。电刺激（0.5毫安，50赫兹，15-25秒）的AV 3V区域的哺乳期大鼠诱发乳房内压力相当于0.25-0.5 μ。催产素（i. v.）。循环中催产素浓度的增加，如通过特定的放射免疫测定法所确定的，证实了AV 3V刺激在哺乳期和非哺乳期大鼠中释放催产素。AV 3V区域的电刺激诱发的血浆催产素浓度的增加依赖于刺激的强度和频率以及电极位置。在25 Hz和0.1 mA刺激后，反应显著增加（P <0.05），在50 Hz和1.0 mA刺激时获得最大反应。刺激正中核及其周围的区域，催产素分泌量最大。射乳反射没有被取消后，急性电解消融的AV 3V区域的麻醉哺乳期大鼠，但电解损伤的AV 3V区域防止血浆催产素浓度的增加，通常遵循的渗透刺激（1毫升1.5摩尔NaCl/升，腹腔注射）。这些研究提供的证据表明，AV 3V区域是催产素神经元的兴奋性传入的主要来源，这种输入是必不可少的，这些神经元的反射性反应，但在控制这些神经元在反射性牛奶喷射的作用不大。
The involvement of the region anterior and ventral to the third ventricle (AV3V region) in the control of oxytocin release was investigated using electrical stimulation and electrolytic lesioning techniques in the rat. Electrical stimulation (0.5 mA, 50 Hz, 15-25 s) of the AV3V region of lactating rats evoked a reproducible rise in intramammary pressure equivalent to that induced by 0.25-0.5 mu. oxytocin (i.v.). Increases in circulating concentrations of oxytocin, as determined by specific radioimmunoassay, confirmed that AV3V stimulation released oxytocin in both lactating and non-lactating rats. The increases in plasma oxytocin concentration evoked by electrical stimulation of the AV3V region were dependent upon intensity and frequency of stimulation, and electrode position. A significant (P less than 0.05) increase followed stimulation at 25 Hz and 0.1 mA, and a maximal response was obtained with 50 Hz and 1.0 mA. Stimulation of the area in and around the nucleus medianus produced the greatest rise in oxytocin secretion. The milk-ejection reflex was not abolished after acute electrolytic ablation of the AV3V region in urethane-anaesthetized lactating rats, but electrolytic lesion of the AV3V region prevented the increase in plasma oxytocin concentration which normally followed an osmotic stimulus (1 ml 1.5 mol NaCl/l, i.p.). These studies provide evidence that the AV3V region is a major source of excitatory afferents to oxytocin neurones; this input is essential for the osmoresponsiveness of these neurones but plays little role in the control of such neurones during reflex milk ejection.