Research note: effect of in ovo 17-beta-estradiol or tamoxifen administration on sexual differentiation of the external genitalia.
Research note: effect of in ovo 17-beta-estradiol or tamoxifen administration on sexual differentiation of the external genitalia.
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研究报告：卵内 17-β-雌二醇或他莫昔芬给药对外生殖器性别分化的影响。
DOI：
10.3382/ps.0711947
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发表时间：
1992
期刊：
影响因子：
4.4
通讯作者：
P. Hargis
中科院分区：
文献类型：
--
作者：
C. Coco;B. Hargis;P. Hargis
研究了他莫昔芬或 17β-雌二醇的卵内给药对鸡外性二态性的影响。在两项试验中，受精卵在孵化第 1 天注射他莫昔芬（每个鸡蛋 200 微克）或媒介物（100 微升玉米油），或在孵化第 11 天注射 17 β-雌二醇（每个鸡蛋 20 微克）或媒介物。通过目视检查外生殖器并在孵化后第 1 天通过性腺鉴定来确定性别。他莫昔芬注射导致表型男性鉴定的数量显着增加，根据外生殖器表型，男性：女性比例为76:24（试验1）和62:38（试验2）。性腺性别鉴定将这些比例纠正为 46:54 和 44:56，导致生殖器性别鉴定错误分别为 27%（试验 1）和 18%（试验 2）。这些误差显着高于用赋形剂处理的雏鸡的生殖器性别鉴定误差（试验 1 和 2 分别为 2% 和 0.6%）。卵内给予 17 β-雌二醇导致基于生殖器性别测定的女性鉴定数量增加，男女比例为 37:63（试验 1）和 46:54（试验 2）。性腺性别鉴定将这些比例纠正为 54:46 和 51:49，导致试验 1 和 2 的生殖器性别鉴定误差分别为 10% 和 6%。这些错误明显高于媒介物处理雏鸡的生殖器性别错误（试验 1 和 2 分别为 4 % 和 0.9%）。本研究的结果表明，早期胚胎施用雌激素或雌激素拮抗剂会改变鸡在孵化时的外性二态性。
The effect of in ovo administration of tamoxifen or 17 beta-estradiol on external sexual dimorphism in chickens was investigated. In two trials, fertile eggs were injected with either tamoxifen (200 micrograms per egg) or vehicle (100 microL corn oil) on Day 1 of incubation, or with 17 beta-estradiol (20 micrograms per egg) or vehicle on Day 11 of incubation. Sexes were determined by visual inspection of the external genitalia and by gonadal identification at Day 1 posthatch. Tamoxifen injection resulted in a significantly greater number of phenotypic male identifications, with male:female ratios of 76:24 (Trial 1) and 62:38 (Trial 2) based on external genitalia phenotype. Gonadal sexing corrected these ratios to 46:54 and 44:56, resulting in genital sexing errors of 27% (Trial 1) and 18% (Trial 2). These errors were significantly higher than genital sexing errors of the chicks treated with vehicle (2 and .6% for Trials 1 and 2, respectively). In ovo administration of 17 beta-estradiol resulted in an increased number of female identifications based on genital sex determination, with male:female ratios of 37:63 (Trial 1) and 46:54 (Trial 2). Gonadal sexing corrected these ratios to 54:46 and 51:49, resulting in genital sexing errors of 10 and 6% for Trials 1 and 2, respectively. These errors were significantly higher than genital sexing errors of vehicle-treated chicks (4 and .9% for Trials 1 and 2, respectively). The results of the present study indicate that early embryonic administration of estrogen or an estrogen antagonist alters chicken external sexual dimorphism near the time of hatch.