Adaptation to hydrogen peroxide enhances PC12 cell tolerance against oxidative damage
Adaptation to hydrogen peroxide enhances PC12 cell tolerance against oxidative damage
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DOI：
10.1016/j.neulet.2005.04.022
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发表时间：
2005-08-05
影响因子：
2.5
通讯作者：
Niki, E
中科院分区：
文献类型：
--
作者：
Chen, ZH;Yoshida, Y;Niki, E
使用PC 12细胞研究了对H2 O2的适应性反应和由此产生的抗氧化应激的保护作用。亚致死剂量H_2O_2预处理对PC 12细胞的毒性有明显的保护作用。内源性抗氧化防御系统，过氧化氢酶和谷胱甘肽过氧化物酶，迅速增强低剂量的H2 O2的预处理。这种预处理也对6-羟基多巴胺和pairaquat诱导的氧化损伤产生保护作用，但对烷基过氧自由基没有保护作用。我们的研究结果表明，低剂量H2 O2的刺激丰富了细胞的抗氧化防御系统，从而增强了细胞对即将到来的H2 O2和相关的羟基自由基诱导的氧化损伤的耐受性。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
The adaptive responses to H2O2 and the resulting protective effect against oxidative stress have been investigated using PC12 cells. Pretreatment of sublethal doses of H2O2 significantly protected PC12 cells against the cytotoxicity induced by lethal H2O2. The endogenous antioxidant defense systems, catalase and glutathione peroxidase, were enhanced quickly by the pretreatment of low doses of H2O2. This pretreatment also exerted protective effect against the oxidative insults induced by 6-hydroxydopamine and pairaquat, but not against alkyl peroxyl radicals. Our results, taken together, suggest that the stimulation by low dose of H2O2 enriches the cellular antioxidant defense systems, thereby enhancing cell tolerance against the forthcoming oxidative insults induced by H2O2 and related hydroxyl radicals. (c) 2005 Elsevier Ireland Ltd. All rights reserved.