Visualization of an AAF induced frameshift mutation: molecular views of base displacement in B-DNA from minimized potential energy calculations.
Visualization of an AAF induced frameshift mutation: molecular views of base displacement in B-DNA from minimized potential energy calculations.
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AAF 诱导移码突变的可视化：通过最小化势能计算得出 B-DNA 中碱基位移的分子视图。
DOI：
10.1093/nar/15.16.6539
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发表时间：
1987
影响因子：
14.9
通讯作者：
Hingerty,BE
中科院分区：
文献类型：
--
作者：
Broyde,S;Hingerty,BE
提出了 AAF 修饰的 B-DNA 十二聚体中碱基位移的能量最小化结构。已采用合理的搜索策略，从修饰的脱氧二核苷单磷酸的构象空间的全局搜索开始，并通过计算机图形学建立模型。已经定位了许多不同的最小能量构象，揭示了碱基移位结构。这些显示了芴链间堆积、芴链间和链内堆积以及位于变性凸起中的非堆积芴。局部螺旋轴有不同程度的不同形式的弯曲，并且一两个碱基对完全变性。一个特别令人感兴趣的结构提供了一种分子观点，表明 AAF 如何诱导在 AAF 修饰的大肠杆菌 (18) 中观察到的 -2 缺失突变。
Energy minimized structures of base displacement in an AAF modified B-DNA dodecamer are presented. A rational search strategy, beginning with a global search of the conformation space of the modified deoxydinucleoside monophosphate, together with model building by computer graphics, has been employed. A number of different minimum energy conformations have been located which reveal base displaced structures. These show fluorene interstrand stacking, fluorene inter- and intrastrand stacking, and non-stacked fluorene situated in the denatured bulge. The local helix axis is bent to various extents in the different forms, and one or two base pairs are fully denatured. One structure of especial interest offers a molecular view that suggests how AAF can induce the -2 deletion mutation observed in AAF modifiedE.coli(18).