Fluorochrome Uvitex 2B Stain for Detection of the Microsporidian Causing Beko Disease of Yellowtail and Goldstriped Amberjack Juveniles
Fluorochrome Uvitex 2B Stain for Detection of the Microsporidian Causing Beko Disease of Yellowtail and Goldstriped Amberjack Juveniles
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荧光 Uvitex 2B 染色剂用于检测引起黄尾鰤和金条鰤幼鱼 Beko 病的微孢子虫
DOI：
10.3147/jsfp.31.99
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发表时间：
1996
期刊：
影响因子：
0.6
通讯作者：
K. Hirano
中科院分区：
文献类型：
--
作者：
H. Yokoyama;Jeong;J. Sato;M. Sano;K. Hirano
用荧光染料Uvitex 2B [4，4-BIS（2-二（2-羟乙基）-氨基-4-（3-磺基苯胺基）-1，3，5-三嗪-6-基氨基）-二苯乙烯-2，2-二磺酸钠盐]染色剂检测引起黄尾五斑Seriola quinqueradiata和黄条琥珀（goldstriped amberjack S.）lalandi青少年。对于寄生虫的检测，显微镜下的荧光检查中的孢子Uvitex 2B染色涂片的躯干肌肉匀浆是更敏感的比传统的视觉检查的“囊肿”在躯干肌肉。石蜡切片的Uvitex 2B-H & E染色适用于检查“包囊”中的孢子形成顺序和孢子扩散到邻近组织或其他器官中。因此，Uvitex 2B染色被认为是有用的，不仅是一个快速和敏感的诊断贝科病，但也为微孢子虫感染的组织病理学研究。
The fluorochrome Uvitex 2B [4, 4-BIS (2-di (2-hyroxyethyl) -amino-4- (3-sulfophenylamino) -1, 3, 5-triazine-6-ylamino) -stilbene-2, 2-disulfonic acid, sodium salt] stain was used for detection of Microsporidium seriolae (Protozoa : Microspora), the causative agent of beko disease of yellowtail Seriola quinqueradiata and goldstriped amberjack S. lalandi juveniles. For detection of the parasite, microscopical fluorescence examination for spores in the Uvitex 2B-stained smears of trunk muscle homogenates was much more sensitive than the conventional visual inspection for “cysts” in the trunk muscle. The Uvitex 2B-H & E stain of deparaffinized sections was applicable to the examination for the sporulation sequence in “cysts” and spore dispersal into adjacent tissues or other organs. Thus, Uvitex 2B stain was found to be useful not only for a rapid and sensitive diagnosis of beko disease but also for histopathological studies of microsporidian infections.