Development and use of 16S rRNA gene targeted PCR primers for the identification of Escherichia coli cells in water
Development and use of 16S rRNA gene targeted PCR primers for the identification of Escherichia coli cells in water
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DOI：
10.1046/j.1365-2672.1998.853535.x
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发表时间：
1998-09-01
影响因子：
4
通讯作者：
Chi, WR
中科院分区：
文献类型：
--
作者：
Tsen, HY;Lin, CK;Chi, WR
确定了致病性和非致病性大肠杆菌菌株的 16S rRNA 基因 V-3 和 V-6 区的一级序列，并与属于肠杆菌科的许多参考菌株获得的一级序列进行了比较。设计了三种寡核苷酸引物 16E1、16E2 和 16E3，并将其用于聚合酶链式反应，以特异性鉴定所有大肠杆菌分离株。当使用 16E1、16E2 和 16E3 作为引物来鉴定自来水、地下水和池塘水中存在的大肠杆菌细胞时，如果在 PCR 反应之前进行 1 h 预培养步骤，则可以检测到低至 I cfu 100 ml(-1) 的水。
The primary sequences of the V-3 and V-6 regions of the 16S rRNA gene of pathogenic and non-pathogenic strains of Escherichia coli were determined and compared with those obtained for a number of reference strains which belong to the family Enterobacteriaceae. Three oligonucleotide primers 16E1, 16E2, and 16E3 were designed and used in the polymerase chain reaction to identify specifically all E. coli isolates. When 16E1, 16E2 ann 16E3 were used as primers for the identification of E. coli cells present in tap, underground and pond waters, as low as I cfu 100 ml(-1) of water could be detected if an a h pre-culture step was performed prior to the PCR reaction.