Isolation of putative progenitor endothelial cells for angiogenesis
Isolation of putative progenitor endothelial cells for angiogenesis
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DOI：
10.1126/science.275.5302.964
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发表时间：
1997-02-14
期刊：
影响因子：
56.9
通讯作者：
Isner, JM
中科院分区：
文献类型：
--
作者：
Asahara, T;Murohara, T;Isner, JM
根据细胞表面抗原的表达，通过磁珠选择从人外周血中分离出假定的内皮细胞（EC）祖细胞或成血管细胞。在体外，这些细胞分化为EC。在局部缺血的动物模型中，异源、同源和自体EC祖细胞掺入活跃血管生成的位点。这些研究结果表明，EC祖细胞可能是有用的，以增加侧支血管生长缺血组织（治疗性血管生成）和提供抗或促血管生成剂，分别到病理或功利性血管生成的网站。
Putative endothelial cell (EC) progenitors or angioblasts were isolated from human peripheral blood by magnetic bead selection on the basis of cell surface antigen expression. In vitro, these cells differentiated into ECs. In animal models of ischemia, heterologous, homologous, and autologous EC progenitors incorporated into sites of active angiogenesis. These findings suggest that EC progenitors may be useful for augmenting collateral vessel growth to ischemic tissues (therapeutic angiogenesis) and for delivering anti- or pro-angiogenic agents, respectively, to sites of pathologic or utilitarian angiogenesis.