AKAP79 increases the functional expression of skeletal muscle Ca2+ channels in Xenopus oocytes.
AKAP79 increases the functional expression of skeletal muscle Ca2+ channels in Xenopus oocytes.
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AKAP79 增加爪蟾卵母细胞骨骼肌 Ca2 通道的功能表达。
DOI：
10.1016/j.bbrc.2004.02.035
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Sanchez,JorgeA
中科院分区：
文献类型：
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作者：
Plata,Consuelo;Escamilla,Juan;Carrillo,Elba;Galindo,JoseM;Gamba,Gerardo;Garcia,MariaC;Sanchez,JorgeA
激酶A锚定蛋白AKAP 79是调节心脏L型钙通道的关键因素，其作用在非洲爪蟾卵母细胞中表达的骨骼肌钙通道上进行了评估。通过表达成孔α 1 s亚基及其辅助亚基α2-δ、β和γ来重建通道。我们首次报道，AKAP 79与截短型α 1 s亚基共表达时，钙通道电流峰值大大增加（3.5倍）。免疫印迹显示，电流幅度的增加并不伴随着α 1 s亚基的膜水平的相应增加。这表明AKAP 79不会增加该通道的运输。此外，我们发现，AKAP 150的转录本，人类AKAP 79的大鼠直系同源物，在大鼠骨骼肌中表达，并提出AKAP 79/150调节Ca 2+通道功能。
The actions of the kinase A anchoring protein, AKAP79, a key element in the regulation of the cardiac L-type Ca2+channel, were assessed on skeletal muscle Ca2+channels expressed in Xenopus oocytes. The channels were reconstituted by expressing the pore forming α1ssubunit and its accessory subunits, α2-δ, β, and γ. We report, for the first time, that peak Ca2+channel currents are greatly increased (3.5-fold) by AKAP79 when co-expressed with the truncated form of the α1ssubunit. Immunoblots revealed that the increase in current amplitude is not accompanied by a corresponding increase in the membrane levels of the α1ssubunit. This suggests that AKAP79 does not increase the trafficking of the channel. In addition, we show that the transcript of AKAP150, the rat ortholog of the human AKAP79, is expressed in rat skeletal muscle and propose that AKAP79/150 modulates Ca2+channel function.