Dissemination of imipenem-resistant Acinetobacter baumannii strains carrying the ISAba1-blaOXA-23 genes in a Chinese hospital
Dissemination of imipenem-resistant Acinetobacter baumannii strains carrying the ISAba1-blaOXA-23 genes in a Chinese hospital
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DOI：
10.1099/jmm.0.47206-0
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发表时间：
2007-08-01
影响因子：
3
通讯作者：
Zheng, Shu-Sen
中科院分区：
文献类型：
--
作者：
Zhou, Hua;Pi, Bo-Rui;Zheng, Shu-Sen
对中国某医院95株耐亚胺培南鲍曼不动杆菌临床感染暴发进行了调查，并对其中的碳青霉烯酶编码基因及其与ISAba 1的关系进行了分析，并对从重症监护病房（ICU）环境中回收的另外16株菌株进行了分析。几乎所有分离株对多种抗菌药物均耐药;多粘菌素E（117.1%）、头孢哌酮/舒巴坦（30.6%）和氨苄西林/舒巴坦（67.6%）的耐药率最低。在临床分离株中，通过DNA宏观限制性酶切图谱确定了6种图谱类型，其中在7个医院单位的患者中传播了A型（50株分离株），在8个单位传播了B型（35株分离株）; ICU的环境分离株也属于A型。所有分离株的bla（OXA-66）和bla（OXA-23）基因均为阳性。OXA-23编码基因位于ISAba 1下游34 bp处。未检测到质粒，也未证实耐药性的接合转移。bla（OXA-23）探针分别与A型和B-1型的200和220 kb Apal染色体片段杂交。
An outbreak of 95 clinical infections with imipenem-resistant Acinetobacter baumannii in a Chinese hospital was investigated and the carbapenemase-encocling genes and their relationship with ISAba 1 of these and a further 16 isolates recovered from the intensive care unit (ICU) environment were analysed. Almost all isolates were resistant to a wide range of antimicrobials; the lowest resistance rates were found for polymyxin E (117.1 %), cefoperazone/sulbactarn (30.6 %) and ampicillin/sulbactam (67.6 %). Six pattern types defined by DNA macrorestriction patterns were distinguished among the clinical isolates with dissemination of pattern A (50 isolates) to patients in seven hospital units and pattern B (35 isolates) to eight units; the environmental isolates from ICUs were also of pattern A. All isolates were positive for the bla(OXA-66) and bla(OXA-23) genes. The OXA-23-encoding gene was located 34 bp downstream of ISAba1. No plasmids were detected and conjugal transfer of resistance was not demonstrated. The bla(OXA-23) probe hybridized with 200 and 220 kb Apal chromosomal fragments for type patterns A and B-1 respectively.