Validation of an HPLC analytical method coupled to a multifunctional clean-up column for the determination of deoxynivalenol
Validation of an HPLC analytical method coupled to a multifunctional clean-up column for the determination of deoxynivalenol
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DOI：
10.1007/s11046-006-0260-1
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发表时间：
2006-04-01
期刊：
影响因子：
5.5
通讯作者：
Takatori, K
中科院分区：
文献类型：
--
作者：
Sugita-Konsihi, Y;Tanaka, T;Takatori, K
为了评价多功能净化柱结合高效液相色谱作为官方分析方法测定食品和饲料用小麦中脱氧雪腐镰刀菌烯醇的方法，对12个实验室进行了实验室间的研究，使用了4个天然污染的小麦样品和1个添加样品。天然污染小麦的重复性(RSDR)和再现性(RSDR)的相对标准偏差分别为5.8~11.3%和12.0~20.7%。每个样本的HORRAT均小于1.0。加标试验回收率为100.0%，RSDR为11.2%，RSDR为10.3%，HORRAT值为0.5。该方法的检出限为0.10 mg/kg，线性校正范围为0.10 mg/kg。因此，对于食品和饲料用小麦中脱氧雪腐镰刀菌烯醇的测定，它应该是一种灵敏和有效的分析方法。
To evaluate a method using a multifunctional clean-up column coupled with high performance liquid chromatography as an official analytical method for the determination of deoxynivalenol in wheat used as food or feed, an inter-laboratory study was performed in 12 laboratories using four naturally contaminated wheat samples and one spiked sample. The relative standard deviations for repeatability (RSDr) and reproducibility (RSDR) of naturally contaminated wheat were in the range 5.8-11.3% and 12.0-20.7%, respectively. The HORRAT was less than 1.0 in each sample. From the spiking test, the recovery rate, RSDr, RSDR and HORRAT value were 100.0%, 11.2%, 10.3% and 0.5, respectively. The limit of quantification is 0.10 mg/kg from the range obtained in a linear calibration. Thus, it should be useful as a sensitive and validated analytical method for the determination of deoxynivalenol in wheat intended for use in food and feed.