Clinical grade manufacturing of human alloantigen-reactive regulatory T cells for use in transplantation.
Clinical grade manufacturing of human alloantigen-reactive regulatory T cells for use in transplantation.
复制标题
DOI：
10.1111/ajt.12433
复制
发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Tang Q
中科院分区：
文献类型：
--
作者：
Putnam AL;Safinia N;Medvec A;Laszkowska M;Wray M;Mintz MA;Trotta E;Szot GL;Liu W;Lares A;Lee K;Laing A;Lechler RI;Riley JL;Bluestone JA;Lombardi G;Tang Q
Regulatory T cell (Treg) therapy has the potential to induce transplantation tolerance so that immunosuppression and associated morbidity can be minimized. Alloantigen-reactive Tregs (arTregs) are more effective at preventing graft rejection than polyclonally expanded Tregs (PolyTregs) in murine models. We have developed a manufacturing process to expand human arTregs in short-term cultures using good manufacturing practice-compliant reagents. This process uses CD40L-activated allogeneic B cells to selectively expand arTregs followed by polyclonal restimulation to increase yield. Tregs expanded 100- to 1600-fold were highly alloantigen reactive and expressed the phenotype of stable Tregs. The alloantigen-expanded Tregs had a diverse TCR repertoire. They were more potent than PolyTregs in vitro and more effective at controlling allograft injuries in vivo in a humanized mouse model.
登录
查看更多内容
DOI：
10.4049/jimmunol.181.4.2855
发表时间：
2008-08-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Golovina TN;Mikheeva T;Suhoski MM;Aqui NA;Tai VC;Shan X;Liu R;Balcarcel RR;Fisher N;Levine BL;Carroll RG;Warner N;Blazar BR;June CH;Riley JL
通讯作者：
Riley JL
影响因子：
16.2
作者：
Marek-Trzonkowska N;Mysliwiec M;Dobyszuk A;Grabowska M;Techmanska I;Juscinska J;Wujtewicz MA;Witkowski P;Mlynarski W;Balcerska A;Mysliwska J;Trzonkowski P
通讯作者：
Trzonkowski P
影响因子：
15.9
作者：
Novak, EJ;Liu, AW;Kwok, WW
通讯作者：
Kwok, WW
影响因子：
20.3
作者：
Veerapathran, Anandharaman;Pidala, Joseph;Anasetti, Claudio
通讯作者：
Anasetti, Claudio
影响因子：
4.4
作者：
Parry, RV;Rumbley, CA;Riley, JL
通讯作者：
Riley, JL