Taxonomer: an interactive metagenomics analysis portal for universal pathogen detection and host mRNA expression profiling.
Taxonomer: an interactive metagenomics analysis portal for universal pathogen detection and host mRNA expression profiling.
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DOI：
10.1186/s13059-016-0969-1
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发表时间：
2016-05-26
期刊：
影响因子：
12.3
通讯作者：
Schlaberg R
中科院分区：
文献类型：
--
作者：
Flygare S;Simmon K;Miller C;Qiao Y;Kennedy B;Di Sera T;Graf EH;Tardif KD;Kapusta A;Rynearson S;Stockmann C;Queen K;Tong S;Voelkerding KV;Blaschke A;Byington CL;Jain S;Pavia A;Ampofo K;Eilbeck K;Marth G;Yandell M;Schlaberg R
High-throughput sequencing enables unbiased profiling of microbial communities, universal pathogen detection, and host response to infectious diseases. However, computation times and algorithmic inaccuracies have hindered adoption. We present Taxonomer, an ultrafast, web-tool for comprehensive metagenomics data analysis and interactive results visualization. Taxonomer is unique in providing integrated nucleotide and protein-based classification and simultaneous host messenger RNA (mRNA) transcript profiling. Using real-world case-studies, we show that Taxonomer detects previously unrecognized infections and reveals antiviral host mRNA expression profiles. To facilitate data-sharing across geographic distances in outbreak settings, Taxonomer is publicly available through a web-based user interface. Taxonomer enables rapid, accurate, and interactive analyses of metagenomics data on personal computers and mobile devices. The online version of this article (doi:10.1186/s13059-016-0969-1) contains supplementary material, which is available to authorized users.
