Expression of puroindoline a enhances leaf rust resistance in transgenic tetraploid wheat
Expression of puroindoline a enhances leaf rust resistance in transgenic tetraploid wheat
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DOI：
10.1007/s11033-007-9070-x
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发表时间：
2008-06-01
影响因子：
2.8
通讯作者：
He, Guangyuan
中科院分区：
文献类型：
--
作者：
Luo, Liting;Zhang, Jinrui;He, Guangyuan
编码嘌呤吲哚啉蛋白（PIN）的嘌呤吲哚啉基因（pin）位于染色体5D上，控制谷物硬度，并且PIN对革兰氏阳性（G（+））细菌、革兰氏阴性（G（-））细菌和真菌具有体外抗微生物活性。小麦叶锈病是由小麦柄锈菌（Pucciniatriticina）引起的小麦重要病害之一。利用基因枪法将puroudindoline a（pinA）基因转化四倍体小麦品种Luna和Venusia，使其组成性表达PINA。在温室和田间试验中，转基因植株对叶锈病的反应增强。收获参数的比较研究表明，转基因植株和对照植株之间存在显着差异。这些指标明显低于对照植物比转基因植物生长更快（P < 0.05），表明它们受到pinA基因的显著影响，并且puroindoline a蛋白（PINA）可以有效抑制体内真菌的生长，转基因四倍体小麦可以在中国中部湖北省生长良好。其中四倍体小麦品种Luna和Venusia由于它们的疾病敏感性而具有低产量。
The purouindoline gene (pin) coding for puroindoline proteins (PINs) is located on chromosome 5D, controls grain hardness, and the PINs have in vitro antimicrobial activity against gram-positive (G(+)) bacteria, gram-negative (G(-)) bacteria and fungi. Wheat leaf rust caused by Puccinia triticina is one of the most important fungal diseases for common wheat with AABBDD genomes. Tetraploid wheat (AABB genome) varieties Luna and Venusia were transformed with the purouindoline a (pinA) gene by bombardment, express PINA consititutively. Transgenic plants showed enhanced response to leaf rust in greenhouse and field. Comparative study of harvesting parameters showed significant differences between transgenic and control plants. These indexes were significantly lower (P < 0.05) in control plants than that in transgenic plants, which suggests that they are significantly affected by pinA gene and that the puroindoline a protein (PINA) can effectively inhibit in vivo the growth of fungal, and the transgenic tetraploid wheat can grow well in Hubei Province, Central China, where the tetraploid wheat varieties Luna and Venusia have poor yield due to their disease-sensitivity.