Morphological effects of mitomycin C on urothelial responses to experimentally-induced urethral stricture in rats
Morphological effects of mitomycin C on urothelial responses to experimentally-induced urethral stricture in rats
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DOI：
10.1111/iju.12780
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发表时间：
2015-07-01
影响因子：
2.6
通讯作者：
Yoon, Sang Pil
中科院分区：
文献类型：
--
作者：
Chang, In Youb;Kim, Jin Nam;Yoon, Sang Pil
目的分析大鼠尿道损伤后尿路上皮对丝裂霉素治疗的反应，认为尿路上皮可能在尿道狭窄的发病机制中起一定作用。方法将sd - dawley大鼠随机分为4组，每组5只：阴性对照组、阳性对照组、透明质酸钠和羧甲基纤维素钠治疗组、经尿道切开后给予丝裂霉素治疗组。结果与阴性对照组相比，阳性对照组尿路上皮细胞增殖和DNA损伤明显增加，DNA修复能力明显下降，导致尿道狭窄。实验对照组与阴性对照组相比，细胞增殖、DNA损伤和DNA修复显著增加。与阳性和实验对照组相比，丝裂霉素处理组细胞增殖和DNA损伤明显减少，但DNA修复明显增加。尿道损伤后DNA损伤立即增加，但丝裂霉素治疗后DNA修复和细胞增殖表达延迟且上调。结论smitomycin可诱导健康的尿路上皮再生，且对尿路上皮无严重损伤。这一发现可能支持丝裂霉素作为尿道狭窄辅助治疗的可能性，也可能提示尿路上皮在尿道损伤后尿道狭窄过程中的作用。
ObjectivesTo analyze the urothelial responses to mitomycinC treatment after urethral injury in rats, as the urothelium might play a role in the pathogenesis of urethral stricture.MethodsMale Sprague-Dawley rats were divided into four groups (n=5/group): negative control, positive control without further treatment, experimental control treated with sodium hyaluronate and sodium carboxymethylcellulose, and experimental treated with mitomycinC after internal urethrotomy.ResultsCompared with negative controls, positive controls showed a significant increase in cell proliferation and DNA damage accompanied by a considerable decrease in DNA repair in the urothelium, which resulted in urethral stricture. Experimental controls showed a significant increase in cell proliferation, DNA damage and DNA repair compared with negative controls. The mitomycinC-treated group showed a significant decrease in cell proliferation and DNA damage, but a considerable increase in DNA repair compared with the positive and experimental control groups. DNA damage was immediately increased after urethral injury, but DNA repair and cell proliferation showed belated and upregulated expression after mitomycinC treatment.ConclusionsMitomycinC could induce healthy re-epithelialization without severe damage in the urothelium. This finding might support the possibility of using mitomycinC as an adjuvant therapy for urethral strictures, and it might also suggest a urothelial role in the process of urethral stricture after urethral injury.