Increased T cell immunoglobulin and mucin domain containing 4 (TIM-4) is negatively correlated with serum concentrations of interleukin-1β in type 2 diabetes
Increased T cell immunoglobulin and mucin domain containing 4 (TIM-4) is negatively correlated with serum concentrations of interleukin-1β in type 2 diabetes
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2 型糖尿病患者 T 细胞免疫球蛋白和粘蛋白结构域 4 (TIM-4) 增加与血清白细胞介素 1 β 浓度呈负相关
DOI：
10.1111/1753-0407.12276
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发表时间：
2016-03-01
影响因子：
4.5
通讯作者：
Gao, Lifen
中科院分区：
文献类型：
--
作者：
Zhao, Peiqing;Wang, Hongxing;Gao, Lifen
研究背景T细胞免疫球蛋白和粘蛋白结构域4（TIM-4）是一种新型免疫调节剂，选择性表达于抗原呈递细胞，特别是巨噬细胞和成熟树突状细胞上。尽管TIM-4在多种免疫疾病中发挥着关键作用，但它是否与2型糖尿病（T2D）有关仍不清楚。本研究的目的是探讨TIM-4在T2D中的表达情况并确定其在疾病进展中的意义。方法从T2D患者和健康对照者中分离外周血单核细胞（PBMC），通过实时聚合酶链反应（PCR）检测TIM-4mRNA的表达，并收集血清测定白细胞介素（IL）-1浓度和其他临床指标（高灵敏度） C反应蛋白[hsCRP]、总胆固醇、低密度脂蛋白胆固醇[LDL-C]、高密度脂蛋白胆固醇、甘油三酯、空腹血糖、HbA1c、天冬氨酸转氨酶和丙氨酸转氨酶）。 结果 PBMCs中TIM-4mRNA的表达显着增加 T2D 患者与健康对照者进行比较。 TIM-4mRNA表达量与血清hsCRP浓度呈正相关。然而，TIM-4mRNA 表达与 IL-1 浓度之间存在负相关，表明 TIM-4 负调节 IL-1 产生的潜在作用。此外，TIM-4mRNA表达与低LDL-C呈负相关，并且TIM-4mRNA表达与HbA1c之间存在负相关趋势。结论本研究的结果表明，TIM-4可能通过调节IL-1而至少部分地促进T2D的发病机制。
BackgroundT cell immunoglobulin and mucin domain containing 4 (TIM-4), a novel immune regulator, is selectively expressed on antigen-presenting cells, especially macrophages and mature dendritic cells. Although TIM-4 plays key roles in mutiple immune diseases, whether it is involved in type 2 diabetes mellitus (T2D) remains unknown. The aim of the present study was to investigate the expression of TIM-4 in T2D and determine its significance in disease progression.MethodsPeripheral blood mononuclear cells (PBMC) were isolated from T2D patients and healthy controls to measure TIM-4mRNA expression by real-time polymerase chain reaction (PCR), and sera were collected to determine interleukin (IL)-1 concentrations and other clinical indicators (high-sensitivity C-reactive protein [hsCRP], total cholesterol, low-density lipoprotein cholesterol [LDL-C], high-density lipoprotein cholesterol, triglyceride, fasting glucose, HbA1c, aspartate aminotransferase, and alanine aminotransferase).ResultsExpression of TIM-4mRNA was increased significantly in PBMCs from T2D patients compared with healthy controls. There was a positive correlation between TIM-4mRNA expression and serum concentrations of hsCRP. However, there was a negative correlation between TIM-4mRNA expression and IL-1 concentrations, indicating the potential role for TIM-4 to negatively regulate IL-1 production. In addition, TIM-4mRNA expression was negatively correlated with lowLDL-C, and there was a tendency for a negative relationship between TIM-4mRNA expression and HbA1c.ConclusionsThe results of the present study indicate that TIM-4 contributes, at least in part, to the pathogenesis of T2D, possibly by regulating IL-1.