Microarray analysis of microRNA expression in peripheral blood cells of systemic lupus erythematosus patients
Microarray analysis of microRNA expression in peripheral blood cells of systemic lupus erythematosus patients
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DOI：
10.1177/0961203307084158
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发表时间：
2007-01-01
期刊：
影响因子：
2.6
通讯作者：
Yin, Y-B
中科院分区：
文献类型：
--
作者：
Dai, Y.;Huang, Y-S;Yin, Y-B
MicroRNA (miRNA) 是 21-24 nt 的非编码 RNA 分子，以转录后方式调节靶基因的表达。有证据表明，miRNA 在胚胎发生、细胞分化和人类疾病的发病机制中发挥着重要作用。本研究描述了系统性红斑狼疮 (SLE) 患者和对照患者的 miRNA 谱之间的比较，以进一步了解 SLE 的发病机制。从 23 名 SLE 患者、10 名特发性血小板减少性紫癜患者和 10 名健康对照者的血液样本中分离出外周血单核细胞。 miRNA 微阵列芯片分析鉴定出 16 个在 SLE 中差异表达的 miRNA。芯片结果通过RNA印迹分析得到证实。这项工作表明 miRNA 是潜在的诊断生物标志物和参与 SLE 发病机制的可能因素。
MicroRNAs (miRNAs) are noncoding RNA molecules of 21-24 nt that regulate the expression of target genes in a post-transcriptional manner. Evidence indicates that miRNAs play essential roles in embryogenesis, cell differentiation and pathogenesis of human diseases. This study describes a comparison between the miRNA profile of the systemic lupus erythematosus (SLE) patients and the controls to develop further understanding of the pathogenesis of SLE. Peripheral blood mononuclear cells were isolated from blood samples of 23 SLE patients, 10 idiopathic thrombocytopenic purpura patients and 10 healthy controls. The miRNA microarray chip analysis identified 16 miRNAs differentially expressed in SLE. The chip results were confirmed by northern blot analysis. This work indicates that miRNAs are potential diagnosis biomarkers and probable factors involved in the pathogenesis of SLE.