The genetic diversity of clinical isolates of Malassezia pachydermatis from dogs and cats.
The genetic diversity of clinical isolates of Malassezia pachydermatis from dogs and cats.
复制标题
狗和猫厚皮马拉色菌临床分离株的遗传多样性。
DOI：
10.1080/mmy.39.4.329.334
复制
发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
Atsuhiko Hasegawa
中科院分区：
文献类型：
--
作者：
T. Aizawa;R. Kano;Yuka Nakamura;S. Watanabe;Atsuhiko Hasegawa
采用随机扩增多态性DNA（RAPD）和几丁质合成酶2（CHS2）基因序列分析方法，对110株犬、猫非脂依赖性厚皮马拉色菌临床分离株进行了分子生物学研究。RAPD分析表明，临床分离的M.粗皮病由A、B、C和D四种不同的遗传类型组成。CHS2基因分析结果与RAPD分析结果完全一致。M.从狗和猫的正常外耳、特应性皮炎、跳蚤过敏性皮炎、外耳炎、脓疡和脂溢性皮炎的病变中获得厚皮病。A型包括93株临床分离株和M.厚皮龙A型M.厚皮病起源于各种疾病的病变。它们主要分布在狗和猫的皮肤上。其他类型（B、C和D）主要从外耳炎中分离出来。
Molecular investigation of 110 clinical isolates of non-lipid-dependent Malassezia pachydermatis from dogs and cats was carried out by random amplification of polymorphic DNA (RAPD) and chitin synthase 2 (CHS2) gene sequence analyses. The RAPD analysis indicated that the clinical isolates of M. pachydermatis constituted four distinct genetic types (A, B, C and D). Moreover, the results from CHS2 gene analysis completely agreed with those from the RAPD analyses. The clinical isolates of M. pachydermatis were obtained from normal external ears, lesions of atopic dermatitis, flea allergic dermatitis, otitis externa, pyoderma and seborrheic dermatitidis in dogs and cats. Type A consisted of 93 clinical isolates as well as the ex-neotype strain of M. pachydermatis. The isolates of type A M. pachydermatis originated from lesions of all kinds of diseases. They were predominant on dog and cat skin. The other types, B, C, and D were isolated mainly from otitis externa.