Metabolic signatures provide novel insights toPicrorhiza kurroaadaptation along the altitude in Himalayan region
Metabolic signatures provide novel insights toPicrorhiza kurroaadaptation along the altitude in Himalayan region
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DOI：
10.1007/s11306-020-01698-8
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发表时间：
2020-06-23
期刊：
影响因子：
3.6
通讯作者：
Kumar, Rajiv
中科院分区：
文献类型：
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作者：
Kumari, Manglesh;Joshi, Robin;Kumar, Rajiv
引种沿海拔方向，环境条件差异很大，可能会影响植物的表现和分布。适应这些变化的条件是一个复杂的生物过程，涉及基因、蛋白质和代谢物的重新编程。药用植物沿海拔方向的代谢反应研究较少。目的在本研究中，我们探讨了黄连胡黄连的适应策略。利用代谢组学方法，以器官特异性的方式沿海拔方向。方法从3个海拔高度随机抽取开花期黄皮黄连植株。喜马拉雅地区的3400、3800和4100毫升。利用叶、根和根茎进行了基于LC-MS的非靶向代谢物图谱和糖、氨基酸、苦皮苷及其对应的酚酸的靶向分析。结果共鉴定出220种初级和次级代谢物(P<；0.05)，代表了广泛的代谢物及其空间分布。库罗亚。代谢产物的不同积累表明，源-库碳分配、部分TCA循环、抗坏血酸代谢、嘌呤分解代谢和抢救途径、嘧啶合成、除赤霉素和细胞分裂素抑制外的脂类改变可能是一种适应沿海拔高山环境胁迫的策略。此外，器官和海拔特异性Sms的显著差异反映了次级代谢途径的改变。胡黄连皂苷的显著积聚提示它们可能在糖尿病肾病中起作用。库罗适应。结论本研究为破译被认为与植物适应有关的代谢物的作用提供了一个平台。
Introduction Along the altitude, environmental conditions vary significantly that might influence plant performance and distribution. Adaptation to these changing conditions is a complex biological process that involves reprogramming of genes, proteins and metabolites. The metabolic response of medicinal plants along the altitude has been less explored yet. Objectives In the present study, we investigated the adaptation strategies ofPicrorhiza kurroaRoyle ex Benth. along the altitude in organ specific manner using metabolomic approach. Methods Picrorhiza kurroaplants at flowering stage were randomly sampled from three altitudes viz. 3400, 3800 and 4100 masl in the Himalayan region. Leaf, root and rhizome were used for LC-MS based non-targeted metabolite profiling and targeted analysis of sugars, amino acids, picrosides and their corresponding phenolic acids. Results A total of 220, primary and secondary metabolites (SMs) were identified (p < 0.05) representing an extensive inventory of metabolites and their spatial distribution inP. kurroa. Differential accumulation of metabolites suggests source-sink carbon partitioning, occurrence of partial TCA cycle, ascorbate metabolism, purine catabolism and salvage route, pyrimidine synthesis, lipid alteration besides gibberellins and cytokinin inhibition might be an adaptive strategy to alpine environmental stress along the altitude. Further, marked differences of organ and altitude specific SMs reflect alteration in secondary metabolic pathways. Significant accumulation of picrosides suggests their probable role inP. kurroaadaptation. Conclusion This study provides a platform that would be useful in deciphering the role of metabolites considered to be involved in plant adaptation.