Effect of Vitamin E Deficiency on Rat Brain Monoamine Metabolism
Effect of Vitamin E Deficiency on Rat Brain Monoamine Metabolism
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DOI：
10.1023/a:1020937409855
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发表时间：
1999-10
影响因子：
4.4
通讯作者：
K. Adachi;M. Izumi;T. Mitsuma
中科院分区：
文献类型：
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作者：
K. Adachi;M. Izumi;T. Mitsuma
我们研究了维生素E缺乏对大鼠脑内单胺代谢的影响。分析了用维生素E缺乏饮食喂养24周的雄性Wistar大鼠。在28周时，它们表现出生长速率降低（减少52%）、肌肉萎缩、后肢运动无力和步态障碍。采用高效液相色谱-电极阵列库仑法同时测定脑内单胺及其前体和代谢物的浓度。此外，测定色氨酸羟化酶活性。维生素E缺乏大鼠脑干中的多巴胺（p = 0.009）和5-羟色胺（p = 0.04）水平显著低于对照组，而脑干中的前体酪氨酸（p = 0.0009）和色氨酸（p = 0.0065）水平显著高于对照组。此外，色氨酸羟化酶活性（p = 0.0005）在脑干维生素E缺乏的大脑显着低于对照组。使用非参数检验（Mann-Whitney U检验）进行所有统计学比较。提示维生素E缺乏可能与脑内单胺代谢紊乱有关。
We investigated the effects of vitamin E deficiency on the monoamine metabolism in the rat brain. Male Wistar rats fed on the vitamin E deficient diet for 24 weeks were analyzed. At 28 weeks, they showed a reduced growth rate (52% of reduction), muscle atrophy, a motor weakness of hind limbs and disturbance of gait. The concentrations of monoamines, their precursors and metabolites in the brain were simultaneously determined using high performance liquid chromatography (HPLC) coupled with a coulometric detection with electrode array system. In addition, tryptophan hydroxylase activity was measured. The dopamine (p = 0.009) and serotonin (p = 0.04) levels in the brain stem of vitamin E deficients rats were significantly lower than in the controls, whereas their precursors tyrosine (p = 0.0009) and tryptophan (p = 0.0065) levels in the brain stem were significantly higher than in the controls. Moreover, tryptophan hydroxylase activity (p = 0.0005) in the brain stem of vitamin E deficient brains was significantly lower than in the controls. All statistical comparisons were done using non-parametric tests (Mann-Whitney U test). These results suggest that vitamin E deficiency may play a role in the disturbance of monoamine metabolism in rat brain.