Actin-binding cellular proteins inside human immunodeficiency virus type 1
Actin-binding cellular proteins inside human immunodeficiency virus type 1
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DOI：
10.1006/viro.1999.0075
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发表时间：
2000-01-05
期刊：
影响因子：
3.7
通讯作者：
Henderson, LE
中科院分区：
文献类型：
--
作者：
Ott, DE;Coren, LV;Henderson, LE
宿主蛋白质在人类免疫缺陷病毒1型（HIV-1）病毒体上和病毒体内均被掺入。为了鉴定HIV-1内的细胞蛋白，病毒体制备物通过蛋白酶消化技术处理，该技术去除外部宿主蛋白，从而允许研究病毒内的蛋白。通过免疫印迹、高压液相色谱和蛋白质序列分析来分析处理的HIV-1制剂。这些分析鉴定了HIV-1内的几种细胞蛋白：延伸因子1 α、甘油醛-3-磷酸脱氢酶、HS-1、磷脂酰乙醇胺结合蛋白、Pin1、Lck、Nm23-H1和CD 43的C末端尾。这些蛋白质中有几种是以其完整蛋白质的片段形式被发现的，这些蛋白质似乎是由我们对病毒体、HIV-1蛋白酶或细胞蛋白酶的蛋白酶处理产生的。细胞生物学和生物化学的最新进展已经确定了这些蛋白质中的一些肌动蛋白结合蛋白。这些结果支持的假设，肌动蛋白丝被纳入病毒粒子，并可能提供额外的线索，为理解在组装和出芽过程中病毒和细胞蛋白之间的相互作用。(C)北京大学出版社.
Host proteins are incorporated both on and inside human immunodeficiency virus type 1 (HIV-1) virions. To identify cellular proteins inside HIV-1, virion preparations were treated by a protease-digestion technique that removes external host proteins, allowing for the study of the proteins inside the virus. Treated HIV-1 preparations were analyzed by immunoblot, high-pressure liquid chromatography, and protein sequence analyses. These analyses identified several cellular proteins inside HIV-1: elongation factor 1 alpha, glyceraldehyde-3-phosphate dehydrogenase, HS-1, phosphatidylethanolamine-binding protein, Pin1, Lck, Nm23-H1, and the C-terminal tail of CD43. Several of these proteins were found as fragments of their full-sized proteins that appear to be generated by our protease treatment of the virions, the HIV-1 protease, or a cellular protease. Recent advances in cell biology and biochemistry have identified some of these proteins as actin-binding proteins. These results support the hypothesis that actin filaments are incorporated into the virion and may provide additional clues for the understanding of the interaction between viral and cellular proteins during assembly and budding. (C) 2000 Academic Press.