Innervation of endometrium and myometrium in women with painful adenomyosis and uterine fibroids
Innervation of endometrium and myometrium in women with painful adenomyosis and uterine fibroids
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患有疼痛性子宫腺肌病和子宫肌瘤的女性子宫内膜和子宫肌层的神经支配
DOI：
10.1016/j.fertnstert.2009.03.026
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发表时间：
2010-07-01
影响因子：
6.7
通讯作者：
Lin, Jun
中科院分区：
文献类型：
--
作者：
Zhang, Xinmei;Lu, Bangchun;Lin, Jun
目的：确定是否可以在患有疼痛性子宫肌瘤和子宫腺肌病的女性的子宫内膜和子宫肌层中检测到神经纤维。设计：一项回顾性免疫组织化学研究。地点：一所学术培训医院。患者：37 名患有子宫肌瘤的女性和 29 名患有子宫腺肌病的女性。干预措施：对相邻子宫内膜和子宫肌组织的组织学切片进行染色使用高度特异性的多克隆兔抗蛋白基因产物 9.5 (PGP9.5) 和单克隆小鼠抗神经丝蛋白 (NF) 进行免疫组织化学检查。主要结果指标：使用 PGP9.5 和 NF 通过免疫组织化学染色确定结果。结果：我们在疼痛女性的子宫内膜功能层中检测到 PGP9.5 免疫活性神经纤维，但在无痛女性中未检测到。疼痛女性子宫内膜或子宫肌层基底层的PGP9.5免疫活性神经纤维密度显着增加，而子宫腺肌症和子宫肌瘤女性的PGP9.5免疫活性神经纤维密度无统计学差异。我们在患有子宫腺肌病和子宫肌瘤的女性子宫内膜和子宫肌层的基底层中发现了 NF-免疫活性神经纤维，但没有发现显着差异。结论：这些结果表明，出现在患有疼痛性子宫腺肌病和子宫肌瘤的女性子宫内膜和子宫肌层中的 PGP9.5-免疫活性神经纤维可能在这两种疾病的疼痛产生中发挥作用。 （Fertil Steril (R) 2010；94:730-7。(C) 2010，美国生殖医学会。）
Objective: To determine whether nerve fibers can be detected in the endometrium and myometrium in women with painful uterine fibroids and adenomyosis.Design: A retrospective immunohistochemical study.Setting: An academic training hospital.Patient(s): Thirty-seven women with uterine fibroids and 29 women with adenomyosis.Intervention(s): Histologic sections of contiguous endometrial and myometrial tissues were stained immunohistochemically using the highly specific polyclonal rabbit antiprotein gene product 9.5 (PGP9.5) and monoclonal mouse antineurofilament protein (NF).Main Outcome Measure(s): Results were determined through immunohistochemical staining using PGP9.5 and NF.Result(s): We detected PGP9.5-immunoactive nerve fibers in the functional layer of the endometrium in women with pain but not in women without pain. PGP9.5-immunoactive nerve fiber density in the basal layer of the endometrium or myometrium significantly increased in women with pain, however, PGP9.5-immunoactive nerve fiber density had no statistical differences between women with adenomyosis and uterine fibroids. We identified NF-immunoactive nerve fibers in the basal layer of the endometrium and myometrium in women with adenomyosis and uterine fibroids, but found no significant differences.Conclusion(s): These results suggest that PGP9.5-immunoactive nerve fibers appearing in the endometrium and myometrium of women with painful adenomyosis and uterine fibroids may play a role in pain generation in these two disorders. (Fertil Steril (R) 2010;94:730-7. (C) 2010 by American Society for Reproductive Medicine.)