Daidzein induces bone morphogenetic protein-2 and runt-related transcription 2 on periodontal ligament cells after experimental tooth movement
Daidzein induces bone morphogenetic protein-2 and runt-related transcription 2 on periodontal ligament cells after experimental tooth movement
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DOI：
10.1080/13440241.2020.1839208
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发表时间：
2020-10
影响因子：
0.4
通讯作者：
Mioko Sakurai;Mami Shimizu
中科院分区：
文献类型：
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作者：
Mioko Sakurai;Mami Shimizu
摘要目的分析大豆黄酮对实验性牙移动后骨形态发生蛋白2（BMP-2）和Runx相关转录因子2（Runx 2）表达的影响。材料与方法36只Wistar大鼠随机分为EXTM组和EXTM+大豆苷元组（EXTM+DZ组）。EXTM+DZ组于造模后给予大豆黄酮10 mg/kg/d灌胃。微电脑断层扫描（CT）分析显示骨密度（BMD）、骨体积/组织体积（BV/TV）和复发率。免疫组化法检测BMP-2和Runx 2的表达。人PDLCs经受张力并用10 μg/mL大豆苷元处理。实时荧光定量聚合酶链反应和酶联免疫吸附试验证实大豆黄酮对BMP-2和Runx 2的表达。结果EXTM+DZ组较EXTM组复发率明显降低，BMD和BV/TV明显增加。EXTM+DZ组BMP-2和Runx-2阳性细胞比例高于EXTM组。大豆苷元还以时间依赖性方式上调BMP-2和Runx 2的mRNA表达和蛋白水平。结论大豆黄酮可能通过促进BMP-2和Runx 2的表达而降低复发率。
ABSTRACT Purpose To analysis, daidzein increases the expression of bone morphogenetic protein-2 (BMP-2) and runt-related transcription factor 2 (Runx2) after experimental tooth movement (EXTM). Materials and Methods 36 Wistar rats were randomly divided into the EXTM group and EXTM with daidzein group (EXTM+DZ group). After EXTM, the rats in the EXTM+DZ group were treated with daidzein (10 mg/kg/day). Micro-computed tomography (CT) analysis revealed the bone mineral density (BMD), ratio of bone volume to tissue volume (BV/TV), and ratio of relapse. Histopathological changes were examined by immunohistochemical staining for BMP-2 and Runx2. Human PDLCs were subjected to tension force and treated with 10 μg/mL daidzein. The expression of BMP-2 and Runx2 by daidzein was confirmed by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Results The EXTM+DZ group showed a significantly decreased ratio of relapse and increased BMD and BV/TV as compared to the EXTM group. The ratio of BMP-2 – and Runx-2 –positive cells in the EXTM+DZ group was higher than that in the EXTM group. Moreover, daidzein upregulated the mRNA expression and protein levels of BMP-2 and Runx2 in a time-dependent manner. Conclusion These results suggested that daidzein decreased the ratio of relapse through BMP-2 and Runx2 expression.