Incidence of HIV infection in monocyte subpopulations characterized by CD4 and HLA-DR surface density.
Incidence of HIV infection in monocyte subpopulations characterized by CD4 and HLA-DR surface density.
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以 CD4 和 HLA-DR 表面密度为特征的单核细胞亚群中 HIV 感染的发生率。
DOI：
10.1097/00002030-199202000-00002
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Fife,KH
中科院分区：
文献类型：
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作者：
Schnizlein-Bick,CT;Sherman,MR;Boggs,DL;Leemhuis,TB;Fife,KH
本研究的目的是确定单核细胞表面CD 4抗原表达与这些细胞感染HIV的频率之间的任何相关性。设计单核细胞表面CD 4表达明显低于CD 4+淋巴细胞。然而，所有的单核细胞表达的HIV CD 4受体和感染的个人有一个显着减少的单核细胞的数量，表达更高密度的CD 4表面fluorescence.MethodsThree-color流式细胞术分析被用来表征单核细胞富集的外周血单核细胞（PBMC）的表面表达的CD 4，CD 14（巨噬细胞抗原），和II类主要组织相容性抗原（HLA-DR）。基于“亮”或“暗”CD 4表面表达，将来自HIV阳性个体的HLA-DR+单核细胞分选成两个亚群。A聚合酶链反应（PCR）检测存在的前病毒HIV序列内的DNA从10 - 5细胞从每个排序population. Results-post-sort分析显示，暗淡的CD 4+单核细胞亚群表达暗淡的HLA-DR表面抗原，而明亮的CD 4+单核细胞亚群包含明亮和暗淡的HLA-DR+细胞。PCR结果显示，四个八暗淡的CD 4+单核细胞亚群含有前病毒的HIV DNA，相比之下，一个八明亮的CD 4+单核细胞subsets.ConclusionsOur数据表明，PBMC表达暗淡的CD 4表面抗原更有可能含有单核细胞的HIV变体比单核细胞表达更高的表面密度的CD 4抗原。
ObjectiveThe purpose of this study was to determine any correlation between the expression of CD4 antigen on the surface of monocytes, and the frequency with which these cells are infected with HIV.DesignCD4 surface expression on monocytes is significantly less than that expressed on CD4+ lymphocytes. Nevertheless, all monocytes express the HIV CD4 receptor and infected individuals have a significant decrease in the number of monocytes that express a higher density of CD4 surface fluorescence.MethodsThree-color flow cytometric analysis was used to characterize monocyte-enriched peripheral blood mononuclear cells (PBMC) in terms of surface expression of CD4, CD14 (macrophage antigen), and class II major histocompatibility antigen (HLA-DR). HLA-DR+ monocytes from HIV-positive individuals were sorted into two subpopulations based on either ‘bright’or ‘dim’CD4 surface expression. A polymerase chain reaction (PCR) assay was used to detect the presence of proviral HIV sequences within the DNA from 10 5 cells from each sorted population.ResultsPost-sort analysis revealed that the dim CD4+ monocyte subset expressed dim HLA-DR surface antigen, while the bright CD4+ monocyte subset contained both bright and dim HLA-DR+ cells. PCR results showed that four out of eight dim CD4+ monocyte subsets contained proviral HIV DNA, compared with one out of eight bright CD4+ monocyte subsets.ConclusionsOur data suggest that PBMC that express dim CD4 surface antigen are more likely to contain monocytotrophic HIV variants than monocytes that express a higher surface density of CD4 antigen.