Ethylene response factors MbERF4 and MbERF72 suppress iron uptake in woody apple plants by modulating rhizosphere pH
Ethylene response factors MbERF4 and MbERF72 suppress iron uptake in woody apple plants by modulating rhizosphere pH
复制标题
乙烯反应因子 MbERF4 和 MbERF72 通过调节根际 pH 值抑制木本苹果植物的铁吸收
DOI：
10.1093/pcp/pcz234
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Han Z. H.
中科院分区：
文献类型：
--
作者：
Zhang G. F.;Liu W.;Feng Y.;Li D. Y.;Li K. T.;Sun Q. R.;Zhai L. M.;Wu T.;Zhang X. Z.;Xu X. F.;Wang Y.;Han Z. H.
缺铁限制了果树的产量。缺铁时，双子叶植物根际H+分泌增加，pH值下降。这导致土壤酸化，促进Fe3+转化为Fe2+，植物可以更好地吸收。本研究探讨了两种抑制性转录因子（乙烯反应因子MbERF4和MbERF72）与H +-ATP酶基因MbHA2之间的关系。以铁低效苹果和铁高效苹果为研究对象。酵母单杂交和电泳迁移率变动分析表明，MbERF 4和MbERF 72都与MbHA 2启动子的GCC盒（AGCCGCC）结合。此外，酵母双杂交和双分子荧光互补分析表明，MbERF4与MbERF72相互作用。此外，β-葡萄糖醛酸酶和荧光素酶报告基因测定表明，MbERF 4和MbERF 72诱导的MbHA2表达抑制是协同的。病毒诱导的MbERF4或MbERF72基因沉默增加了MbHA2的表达，从而降低了M.浆果。因此，缺铁诱导的MbERF 4和MbERF 72的高表达有助于M.浆果。MxERF4和MxERF72的低表达有助于M.通过不同的结合条件与HA2启动子的结合，将HA2启动子与小金藻的DNA结合。总之，本研究确定了两个抑制性转录因子与H +-ATPase基因的关系，并提出了一个模型，其中ERF4和ERF72影响根际pH值响应缺铁。
Iron (Fe) deficiency limits the yield of fruit trees. When subjected to Fe deficiency, H+ secretion increases in the rhizosphere of dicotyledonous plants and pH decreases. This leads to the acidification of the soil and promotes Fe3+ to Fe2+ conversion, which plants can better uptake. This study investigated the relationship between two inhibitory transcription factors (ethylene response factors MbERF4 and MbERF72) and the H+-ATPase gene MbHA2. Two species of apple woody plants were studied: the Fe-inefficient Malus baccata and the Fe-efficient Malus xiaojinensis. Yeast one-hybrid and electrophoretic mobility shift assays showed that both MbERF4 and MbERF72 bind to the GCC cassette (AGCCGCC) of the MbHA2 promoter. Moreover, yeast two-hybrid and bimolecular fluorescence complementation assays showed that MbERF4 interacts with MbERF72. Furthermore, beta-glucuronidase and luciferase reporter assays showed that the MbERF4- and MbERF72-induced repression of MbHA2 expression is synergistic. Virus-induced gene silencing of MbERF4 or MbERF72 increased MbHA2 expression, and thus lowered the rhizosphere pH in M. baccata. Consequently, the high expressions of MbERF4 and MbERF72 induced by Fe deficiency contributed to the Fe sensitivity of M. baccata. Moreover, the low expressions of MxERF4 and MxERF72 contributed to the Fe-deficiency tolerance of M. xiaojinensis via different binding conditions to the HA2 promoter. In summary, this study identified the relationship of two inhibitory transcription factors with the H+-ATPase gene and proposed a model in which ERF4 and ERF72 affect the rhizosphere pH in response to Fe deficiency.