Gibberellin-regulated expression of neutral and vacuolar invertase genes in petioles of sugar beet plants
Gibberellin-regulated expression of neutral and vacuolar invertase genes in petioles of sugar beet plants
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DOI：
10.1016/j.plantsci.2007.01.001
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发表时间：
2007-04-01
期刊：
影响因子：
5.2
通讯作者：
Cejudo, Francisco Javier
中科院分区：
文献类型：
--
作者：
Gonzalez, Maria-Cruz;Cejudo, Francisco Javier
从甜菜植株中分离到一个编码与中性转化酶高度相似的多肽的全长cDNA克隆(BVInv-N)。对BVInv-N基因表达模式的分析表明，BVInv-N基因主要在幼株的叶柄和成熟的主根中表达。成熟植株叶柄中中性转化酶表达的降低与相应活性的降低相关，这表明甜菜中性转化酶具有与生长相关的功能。用赤霉素生物合成抑制剂多效唑处理后，叶柄中BVInv-N和空泡转化酶(BVInv-V3)的转录本数量减少，而细胞壁转化酶(BVInv-CW1和BVInv-CW2)的表达没有受到显著影响。赤霉酸处理逆转了多效唑对可溶性转化酶表达的影响；然而，赤霉酸单独处理对这些基因在叶柄中的表达没有影响，这可能是由于内部气体饱和表达所致，与赤霉酸生物合成有关的GA 20-氧化酶(BVGA20ox)的高转录水平表明。甜菜幼株叶柄的协调发育和赤霉素调控的表达支持中性和液泡转化酶在该组织的活跃生长中的作用。(C)2007爱思唯尔爱尔兰有限公司。保留所有权利。
A full-length cDNA clone (BVInv-N) encoding a polypeptide with high similarity to neutral invertases was isolated from sugar beet (Beta vulgaris) plants. The analysis of the expression pattern of the BVInv-N gene revealed a predominant expression in petioles of juvenile plants and mature tap roots. The decrease in neutral invertase expression in petioles of mature plants correlated with a decrease in the corresponding activity, suggesting a growth related function for neutral invertase in sugar beet plants. Treatment of plants with the gibberellin biosynthesis inhibitor, paclobutrazol, decreased the amount of BVInv-N and vacuolar invertase (BVInv-V3) transcripts in petioles, whereas the expression of cell wall invertases (BVInv-CW1 and BVInv-CW2) was not significantly affected in any of these organs. Gibberellic acid treatment reverted the effect of paclobutrazol on soluble invertase expression; however, gibberellic acid alone had no effect on the expression of these genes in the petioles probably due to saturated expression by internal GAs, as suggested by the high transcript levels of GA 20-oxidase (BVGA20ox), a gene involved in gibberellic acid biosynthesis. The coordinated developmental and gibberellin-regulated expression in petioles of juvenile sugar beet plants supports a role of neutral and vacuolar invertases in the active growth of this tissue. (c) 2007 Elsevier Ireland Ltd. All rights reserved.