Second-harmonic-generation microscope with a microlens array scanner
Second-harmonic-generation microscope with a microlens array scanner
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DOI：
10.1364/ol.27.001324
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发表时间：
2002-08-01
期刊：
影响因子：
3.6
通讯作者：
Kawata, S
中科院分区：
文献类型：
--
作者：
Kobayashi, M;Fujita, K;Kawata, S
我们提出了一种多焦点扫描技术，在二次谐波产生（SHG）显微镜中的SHG检测效率和图像采集速率提高了几十倍，与典型的单焦点扫描技术相比。由于微透镜阵列扫描器，锁模钛宝石激光器的激光束被分成大约100个小光束，并且在样品中形成相同数量的焦点。焦点的平行扫描使我们能够观察标本的实时图像。的成像性能和显微镜的实用性证明了由SHG图像的活HeLa细胞和大鼠心肌细胞。(C)2002年美国光学学会。
We propose a multifocus scanning technique in second-harmonic-generation (SHG) microscopy in which the SHG detection efficiency and the image acquisition rate are improved by several tens of times compared with typical single-focus scanning techniques. Because of a microlens array scanner, the laser beam of a mode-locked Ti:sapphire laser is split into similar to100 beamlets, and the same number of foci are formed in the specimen. Parallel scanning of the foci enables us to observe real-time images of the specimen. The imaging properties and the usefulness of the microscope are demonstrated by SHG images of living HeLa cells and rat cardiac myocytes. (C) 2002 Optical Society of America.