Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals
Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals
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DOI：
10.1016/j.mce.2008.11.017
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发表时间：
2009-11-27
影响因子：
4.1
通讯作者：
Gomez, Ana
中科院分区：
文献类型：
--
作者：
Felip, Alicia;Zanuy, Silvia;Gomez, Ana
卡斯普丁。KiSS-1基因的产物最近在不同的哺乳动物和可能的非哺乳动物物种中成为生殖功能的基本调节因子。到目前为止。在哺乳动物中已经描述了一种单一形式的KISS-1，最近，在几种鱼类和非洲爪哇中，我们报道了硬骨鱼中两个不同的KISS-1和KISS-2基因的克隆和特征。KISS-1编码的多肽与啮齿动物的KISS-10相同，而KISS-2预测的十肽有4个氨基酸(FNFNPFGLRF)。基因组数据库搜索显示，这两个基因确实存在于非胎盘性脊椎动物的基因组中，系统发育和基因组图谱分析表明，KiSS-1和KiSS-2是起源于祖先基因复制的平行基因，尽管KiSS-2在胎盘哺乳动物中缺失。KiSS-1和KiSS-2的mRNAs存在于鲈鱼、青竹和斑马鱼的大脑和性腺中。功能比较研究表明，KiSS-2十肽比KiSS-1多肽更能促进黑鱼体内促黄体生成素和卵泡刺激素的分泌。相比之下，KiSS-2十肽只能微弱地刺激大鼠促黄体生成素的分泌。而KiSS-1多肽的效果最好。我们的数据首次提供了确凿证据，证明在非胎盘脊椎动物中存在第二个KISS基因KISS-2。其产物可能在调节促性腺激素轴方面发挥主要的刺激作用，至少在硬骨鱼中是这样。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利
Kisspeptins. the products of KiSS-1 gene, have recently emerged as fundamental regulators of reproductive function indifferent mammalian and, presumably, non-mammalian species. To date. a single form of KiSS-1 has been described in mammals, and recently, in several fish species and Xenopus We report herein the cloning and characterization of two distinct KiSS-like genes, namely, KiSS-1 and KiSS-2, in the teleost sea bass. While KiSS-1 encodes a peptide identical to rodent kisspeptin-10, the predicted KiSS-2 decapeptide diverges at 4 amino acids (FNFNPFGLRF). Genome database searches showed that both genes are present in non-placental vertebrate genomes Indeed, phylogenetic and genome mapping analyses suggest that KISS-1 and KiSS-2 are paralogous genes that originated by duplication of an ancestral gene, although KiSS-2 is lost in placental mammals. KiSS-1 and KiSS-2 mRNAs are present in brain and gonads of sea bass, medaka and zebrafish. Comparative functional studies demonstrated that KiSS-2 decapeptide was significantly more potent than KiSS-1 peptide in inducing LH and FSH secretion in sea bass. In contrast, KiSS-2 decapeptide only weakly elicited LH secretion in rats. whereas KiSS-1 peptide was maximally effective. Our data are the first to provide conclusive evidence for the existence of a second KiSS gene, KiSS-2, in non-placental vertebrates. whose product is likely to play a dominant stimulatory role in the regulation of the gonadotropic axis at least in teleosts. (C) 2008 Elsevier Ireland Ltd. All rights reserved