REPLACEMENT OF THE PROMOTER OF THE YEAST PLASMA-MEMBRANE ATPASE GENE BY A GALACTOSE-DEPENDENT PROMOTER AND ITS PHYSIOLOGICAL CONSEQUENCES
REPLACEMENT OF THE PROMOTER OF THE YEAST PLASMA-MEMBRANE ATPASE GENE BY A GALACTOSE-DEPENDENT PROMOTER AND ITS PHYSIOLOGICAL CONSEQUENCES
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DOI：
10.1007/bf00434664
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发表时间：
1987-01-01
期刊：
影响因子：
2.5
通讯作者：
SERRANO, R
中科院分区：
文献类型：
--
作者：
CID, A;PERONA, R;SERRANO, R
为了探讨酵母[酿酒酵母]质膜ATP酶的生理作用，我们已经取代了其基因的组成型启动子的半乳糖依赖性启动子。当预先形成的ATP酶被稀释到正常浓度的20%时，所产生的细胞停止在葡萄糖培养基上生长。ATP酶活性与细胞质子流出和氨基酸转运之间存在相关性。大部分生长停滞的细胞出现扩大，并有几个芽含有核。
In order to probe the physiological role of the yeast [Saccharomyces cerevisiae] plasma membrane ATPase we have replaced the constitutive promoter of its gene by a galactose-dependent promoter. The resulting cells stop growing on glucose medium when the preformed ATPase is diluted to 20% of normal. There is a correlation between ATPase activity and both proton efflux from the cells and amino acid transport. A large proportion of growth-arrested cells appear enlarged and with several buds containing nuclei.