Mouse tail vertebrae adapt to cyclic mechanical loading by increasing bone formation rate and decreasing bone resorption rate as shown by time-lapsed in vivo imaging of dynamic bone morphometry
Mouse tail vertebrae adapt to cyclic mechanical loading by increasing bone formation rate and decreasing bone resorption rate as shown by time-lapsed in vivo imaging of dynamic bone morphometry
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DOI：
10.1016/j.bone.2011.08.035
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发表时间：
2011-12-01
期刊：
影响因子：
4.1
通讯作者：
Mueller, Ralph
中科院分区：
文献类型：
--
作者：
Lambers, Floor M.;Schulte, Friederike A.;Mueller, Ralph
已知机械负荷通过骨形成和骨吸收之间的平衡的正向转变导致骨量增加。然而，作为负荷的影响，重塑部位如何随时间变化仍有待澄清。本文的目的是调查如何骨形成和吸收网站调制机械负荷随着时间的推移，通过使用一种新的成像技术，提取三维形成和吸收参数的时间推移在体内的微型计算机断层扫描图像。为了诱导负荷适应，C57 BL/6小鼠的第六尾椎骨通过相邻椎骨中的销以8 N或0 N（对照）每周三次循环加载，持续5分钟（3000个循环），总共4周。结果表明，机械负荷使松质骨体积分数显著增加20%（p
It is known that mechanical loading leads to an increase in bone mass through a positive shift in the balance between bone formation and bone resorption. How the remodeling sites change over time as an effect of loading remains, however, to be clarified. The purpose of this paper was to investigate how bone formation and resorption sites are modulated by mechanical loading over time by using a new imaging technique that extracts three dimensional formation and resorption parameters from time-lapsed in vivo micro-computed tomography images. To induce load adaptation, the sixth caudal vertebra of C57BL/6 mice was cyclically loaded through pins in the adjacent vertebrae at either 8 N or 0 N (control) three times a week for 5 min (3000 cycles) over a total of 4 weeks. The results showed that mechanical loading significantly increased trabecular bone volume fraction by 20% (p