Tumor Microenvironments-Adaptive Apoptotic Effects of Cytidine 5′-monophosphate-Capped Gold Nanoclusters
Tumor Microenvironments-Adaptive Apoptotic Effects of Cytidine 5′-monophosphate-Capped Gold Nanoclusters
复制标题
肿瘤微环境-胞苷5-单磷酸封端的金纳米簇的适应性凋亡作用
DOI：
10.1021/acsabm.2c00380
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发表时间：
2022
影响因子：
4.7
通讯作者：
Yuqing Wu
中科院分区：
文献类型：
--
作者：
Chun-Xia Zhang;Hong-Wei Li;Renwen Zhang;Zhongyuan Ren;Yuqing Wu
在目前的工作中，胞苷5 '-单磷酸封端的金纳米团簇（AuNCs@CMP）被报道作为氧化还原反应的催化剂，其显示出氧化酶和优异的过氧化物酶样活性。以3，3 '，5，5'-四甲基联苯胺（TMB）为底物，在过氧化氢（H2 O2）存在下，最大速度（Vmax）为175 × 10-8 Ms-1。此外，AuNCs@CMP对H2 O2产生活性氧（ROS）具有较高的催化活性。特别地，它们还显示出对肿瘤细胞中ROS生成的优异催化活性，被肿瘤微环境（TME）激活和促进。因此，通过流式细胞术测定，AuNCs@CMP对HeLa和SW 480细胞显示出优异的抗肿瘤作用。AuNCs@CMP的抗肿瘤机制归因于基于TME的特定环境的高ROS产生。因此，本研究提供了具有优异的模拟过氧化物酶活性的TME适应性AuNCs@CMP，产生显著的ROS以杀死TME中的肿瘤细胞。
In the present work, cytidine 5'-monophosphate capped gold nanoclusters (AuNCs@CMP) are reported as a catalyst for redox reactions, which show both oxidase- and excellent peroxidase-like activity. When employing 3,3',5,5'-tetramethylbenzidine (TMB) as a substrate in the presence of hydrogen peroxide (H2O2), the maximum velocity (Vmax) was 175 × 10-8 M s-1in vitro. Besides, the AuNCs@CMP exhibited high catalytic activity for reactive oxygen species (ROS) generation with H2O2. Particularly, they also displayed excellent catalytic activity for ROS generation in tumor cells, being activated and promoted by the tumor microenvironment (TME). Consequently, the AuNCs@CMP show an excellent antitumor effect on HeLa and SW480 cells as assayed by flow cytometry. The antitumor mechanism of AuNCs@CMP was attributed to the high ROS generation based on the specific environments of the TME. Therefore, the present study provides TME-adaptive AuNCs@CMP with excellent mimetic peroxidase activity, producing significant ROS to kill the tumor cells in TME.