Four-wave mixing microscopy: a high potential nonlinear imaging method
Four-wave mixing microscopy: a high potential nonlinear imaging method
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四波混合显微镜：一种高潜力的非线性成像方法
DOI：
10.1117/12.2076743
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Alexander Heisterkamp
中科院分区：
文献类型：
--
作者：
Tobias Ehmke;Andreas Knebl;Alexander Heisterkamp
在这项工作中，我们提出了非共振四波混频显微镜作为一个额外的对比机制，在非线性显微镜。该技术的设置是基于市售的多光子显微镜设置配备有钛：飞秒激光器和光学参量振荡器作为光源。基本的系统特性的时空脉冲重叠和像差的影响的过程中。关于在新鲜猪肉上进行的四波混频信号的方向性的实验显示，向后与向前信号平均强度的平均比率为0.16 ± 0.01。尽管如此，两种检测方式的结构信息具有可比性。这突出了四波混频显微镜在体内应用的潜力。此外，猪肉的结果显示了四波混频产生的额外对比度。总之，研究结果表明，非共振四波混频显微镜作为无标记成像模式在生物医学科学中具有很大的潜力。
In this work we present non-resonant four-wave mixing microscopy as an additional contrast mechanism in nonlinear microscopy. The setup for this technique was based on a commercially available multiphoton microscope setup equipped with a titanium:sapphire-laser and an optical parametric oscillator as light sources. Fundamental system characteristics with respect to the spatio-temporal pulse overlap and the influence of aberrations on the process are presented. Experiments regarding the directionality of the four-wave mixing signal performed on fresh porcine meat showed an average ratio of the backward to forward signal mean intensity of 0.16 ± 0.01. Nevertheless, structural information is comparable for both detection modalities. This highlights the potential of four-wave mixing microscopy for in vivo applications. Furthermore, results on porcine meat show the additional contrast generated by four-wave mixing. In summary, the results show a great potential of non-resonant four-wave mixing microscopy as label-free imaging modality in the biomedical sciences.
影响因子：
3.8
作者：
Olivier, Nicolas;Aptel, Florent;Beaurepaire, Emmanuel
通讯作者：
Beaurepaire, Emmanuel
影响因子：
2.9
作者：
Bioud, Fatma-Zohra;Gasecka, Paulina;Brasselet, Sophie
通讯作者：
Brasselet, Sophie
影响因子：
3.4
作者：
Campagnola, PJ;Wei, MD;Loew, LM
通讯作者：
Loew, LM