Effect of acute exhausting exercise on cytokine gene expression in men.

Effect of acute exhausting exercise on cytokine gene expression in men.
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急性力竭运动对男性细胞因子基因表达的影响。

DOI:
10.1055/s-2007-972850
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发表时间:
1996
期刊:
International journal of sports medicine.
影响因子:
--
通讯作者:
Gause,WL
Gause,WL
中科院分区:
--
文献类型:
--
作者:
Natelson,BH;Zhou,X;Ottenweller,JE;Bergen,MT;Sisto,SA;Drastal,S;Tapp,WN;Gause,WL

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修订后接受:1995年8月28日m# mm由于最近对体育锻炼对免疫功能的影响的兴趣,我们决定使用最先进的方法来评估外周血白细胞(PBk)中的细胞因子。7名男性在最大跑步机负荷试验前后的平均体重。从PBk定量细胞因子基因表达的变化。使用逆转录酶聚合酶链反应测定(RT-PCR)。与血清水平或使用体外试验的报告相反。TNF-α的直接基因表达在应激试验后降低(p < 0. 05)。008)。然而,47%的下降相对较小,生物学意义值得怀疑。IL-1 a、IL-1 B、IL-2的水平。IL-4、IL-6。IL-10。而1 NF-γ没有变化。
Accepted after revision: August 28.1995 m# mm Because of recent interest in the effects of physical exercise on immunologic function, we decided to use state-of-the-art methods to evaluate cytokines in the peripheral blood leukocytes (PBk.) of 7 men before and after a maximal treadmill stress test. Change in cytokine gene expression was quantified from PBk. using a reverse transcriptase polymerase chain reaction assay (RT-PCR). In contrast to reports on serum levels or using In vltro testing. direct gene expression of TNF-a decreased after the stress test (p e0. 008). However, the 47% decrease was relatively small and of questionable biological significance. Levels of IL-la, IL-1 b, IL-2. IL-4, IL-6. IL-10. and 1NF-y did not change.