Common human melanoma-associated antigen(s) detected by monoclonal antibodies.
Common human melanoma-associated antigen(s) detected by monoclonal antibodies.
复制标题
通过单克隆抗体检测常见的人类黑色素瘤相关抗原。
作者：
S. Carrel;R. Accolla;A. Carmagnola;J. Mach
杂交瘤细胞来源于小鼠骨髓瘤细胞（P3-NSI/1Ag 4）与来自用来自人黑素瘤细胞系Me-43的膜富集级分免疫的小鼠的脾细胞之间的融合。在获得的26个杂交体中，有7个分泌的抗体与用于免疫测定的黑素瘤细胞系反应。在16个黑色素瘤细胞系、15个其他肿瘤和14个淋巴母细胞系上进一步研究了由7个阳性杂交体产生的抗体的特异性。来自四个阳性杂交体的抗体显示出广泛的反应性，而来自三个杂交体的抗体仅与黑色素瘤细胞反应。来自这三种杂交体中的两种的抗体，α-Mel/5和α-Mel/14，似乎针对常见的黑素瘤抗原，因为它们与所有（除了一个例外）16种黑素瘤细胞系反应，仅与16种黑素瘤细胞系中的5种反应。使用[3 H]亮氨酸标记的抗体的相互结合抑制试验表明，α-Mel/5和α-Mel/14抗体针对不同的抗原决定簇。
Hybridoma cells have been derived from a fusion between mouse myeloma cells (P3-NSI/1Ag4) and spleen cells from a mouse immunized with membrane-enriched fractions from the human melanoma cell line Me-43. Of the 26 hybrids obtained, seven secreted antibodies which reacted with the melanoma cell line used for immunoassay. The specificity of the antibodies produced by the seven positive hybrids was further investigated on 16 melanoma cell lines, 15 other tumors, and 14 lymphoblastoid cell lines. The antibodies from four positive hybrids showed a broad reactivity, whereas those from three hybrids reacted exclusively with melanoma cells. The antibodies from two of these three hybrids, alpha-Mel/5 and alpha-Mel/14, seem to be directed against common melanoma antigen(s) since they reacted with all (with one exception) of the 16 melanoma cell lines tested only with five of the 16 melanoma lines. Reciprocal binding inhibition tests using [3H]leeucine-labeled antibodies showed that alpha-Mel/5 and alpha-Mel/14 antibodies were directed against different antigenic determinants.