Determination of acetyl-CoA and malonyl-CoA in germinating rice seeds using the LC-MS/MS technique
Determination of acetyl-CoA and malonyl-CoA in germinating rice seeds using the LC-MS/MS technique
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DOI：
10.1271/bbb.60269
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发表时间：
2006-11-01
影响因子：
1.6
通讯作者：
Satoh, Kiyoshi
中科院分区：
文献类型：
--
作者：
Hayashi, Osamu;Satoh, Kiyoshi
建立了测定植物组织中乙酰辅酶A和丙二酰辅酶A含量的高灵敏度、高选择性分析方法。分析方法包括简便的植物样品提取方法和利用离子对试剂的LC-MS/MS新技术。这些酰基辅酶a存在于发芽的水稻种子中，然后用所开发的方法测定。结果表明，在水稻种子萌发过程中，乙酰辅酶a和丙二酰辅酶a的浓度均随时间的增加而增加，并且随着培养温度的升高而增加。这些结果反映了。在发芽的水稻种子中产生这些酰基辅酶a的酶的发育。
A highly sensitive and selective analytical method was developed to determine levels of acetyl-CoA and malonyl-CoA in plant tissues. The analytical method includes a convenient extraction method for plant samples and a new LC-MS/MS technique utilizing an ion pair reagent. These acyl-CoAs, present in germinating rice seeds, were then determined by the method developed. It was found that the concentrations of both acetyl-CoA and malonyl-CoA increased with time during the germination of rice seeds and also increased at the elevated cultivation temperatures among tested. These results reflect the. development of enzymes that produce these acyl-CoAs in germinating rice seeds.