Local synaptic organization of cholinergic neurons in the basolateral hypothalamus.
Local synaptic organization of cholinergic neurons in the basolateral hypothalamus.
复制标题
下丘脑基底外侧胆碱能神经元的局部突触组织。
DOI：
10.1002/cne.902760202
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Hayward,JN
中科院分区：
文献类型：
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作者：
Meeker,RB;Swanson,DJ;Hayward,JN
胆碱乙酰转移酶（ChAT）的单克隆抗体用于免疫细胞化学鉴定的胆碱能神经元在基底外侧下丘脑。光镜和电镜检查发现，视上核背外侧的细胞体，树突和轴突过程的网络。在该区域内，ChAT免疫反应性细胞接受许多未标记的末端，其接触树突、细胞索马、轴突和偶尔的体棘。在少数情况下，观察到小ChAT免疫反应终末接触胆碱能细胞索马或大树突。许多ChAT免疫反应纤维指向视上核，形成致密的局部网络，但这些纤维中很少有穿透到视上核内超过约20 μm。总共有63个ChAT免疫反应终末被映射到基底下丘脑，其中绝大多数接触未标记的树突直接背外侧的视上核。标记的终端是罕见的或不存在的内侧部分的下丘脑或深内的视上核。这种ChAT末端密度的模式与毒蕈碱胆碱能探针[3 H]奎宁环二苯甲酸盐的结合分布相关，但与推定的烟碱胆碱能探针[125 I] α银环蛇毒素的结合无关，后者在视上核内的结合率较高。因此，基底下丘脑的胆碱能神经元似乎形成了一个内在连接的网络，这可能代表了对毒蕈碱胆碱能受体的输入。没有证据表明胆碱能突触前末梢与视上核内的α-银环蛇毒素结合蛋白共定位。
A monoclonal antibody to choline acetyltransferase (ChAT) was utilized for immunocytochemical identification of cholinergic neurons in the basolateral hypothalamus. Light and electron microscopic examination revealed a network of cell bodies, dendrites, and axonal processes dorsolateral to the supraoptic nucleus. Within this region the cells immunoreactive for ChAT receive numerous unlabeled terminals which contact dendrites, cell soma, axons and occasional somatic spines. In a few cases, small ChAT‐immunoreactive terminals were observed contacting a cholinergic cell soma or large dendrite. Many ChAT‐immunoreactive fibers were directed toward the supraoptic nucleus forming a dense local network but very few of these fibers penetrated deeper than approximately 20 μm into the supraoptic nucleus. A total of 63 ChAT‐immunoreactive terminals were mapped within the basal hypothalamus, of which the vast majority contacted unlabeled dendrites immediately dorsolateral to the supraoptic nucleus. Labeled terminals were rare or nonexistent in the medial portions of the hypothalamus or deep within the supraoptic nucleus. This pattern of ChAT terminal densities correlates with the distribution of binding for the muscarinic cholinergic probe, [3H]quinuclidinylbenzilate, but not the binding of the putative nicotinic cholinergic probe, [125I]alpha‐bungarotoxin, which is high within the supraoptic nucleus. Thus, the cholinergic neurons of the basal hypothalamus appear to form a network of intrinsic connections which probably represent input to muscarinic cholinergic receptors. No evidence was found to suggest that cholinergic presynaptic terminals were colocalized with the alpha‐bungarotoxin binding protein within the supraoptic nucleus.