Contact guidance of smooth muscle cells is associated with tension-mediated adhesion maturation
Contact guidance of smooth muscle cells is associated with tension-mediated adhesion maturation
复制标题
平滑肌细胞的接触引导与张力介导的粘附成熟相关
DOI：
10.1016/j.yexcr.2014.05.002
复制
发表时间：
2014
影响因子：
3.7
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Saito;A.C.;Matsui;T.S.;Ohishi;T.;Sato;M.;Deguchi;S.
接触引导是在伤口愈合和发育模式期间观察到的细胞现象，其中粘附细胞由于物理线索而在相同方向上排列。尽管有许多研究，但对细胞取向一致的分子机制知之甚少。在这里，我们制作了间距为亚微米的微槽，以研究平滑肌细胞的接触引导。我们表明，整合素为基础的细胞基质粘附和细胞张力是必要的，以实现接触指导沿着微槽。我们通过对桩蛋白的分析进一步表明，当细胞-基质粘附沿沿着微槽运行时，比与微槽方向成角度排列时，细胞-基质粘附更容易成熟。由于细胞张力促进细胞-基质粘附的成熟，我们提出，与那些沿沿着微槽排列的粘附相比，沿微槽排列的粘附对于承受细胞张力而言不是物理上有效的。因此，所提出的模型描述了一种接触引导机制，即细胞最终将优先沿沿着微槽排列，因为细胞张力更容易在该方向内承受，并且所产生的成熟粘附的方向决定了整个细胞的方向。
Contact guidance is a cellular phenomenon observed during wound healing and developmental patterning, in which adherent cells align in the same direction due to physical cues. Despite numerous studies, the molecular mechanism underlying the consistent cell orientation is poorly understood. Here we fabricated microgrooves with a pitch of submicrons to study contact guidance of smooth muscle cells. We show that both integrin-based cell–substrate adhesions and cellular tension are necessary to achieve contact guidance along microgrooves. We further show through analyses on paxillin that cell–substrate adhesions are more prone to become mature when they run along microgrooves than align at an angle to the direction of microgrooves. Because cellular tension promotes the maturation of cell–substrate adhesions, we propose that the adhesions aligning across microgrooves are not physically efficient for bearing cellular tension compared to those aligning along microgrooves. Thus, the proposed model describes a mechanism of contact guidance that cells would finally align preferentially along microgrooves because cellular tensions are more easily borne within the direction, and the direction of resulting mature adhesions determines the direction of the whole cells.