PROSTAGLANDIN-E2 PRODUCTION BY RABBIT URINARY-BLADDER
PROSTAGLANDIN-E2 PRODUCTION BY RABBIT URINARY-BLADDER
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DOI：
10.1152/ajprenal.1980.239.5.f452
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发表时间：
1980-01-01
影响因子：
--
通讯作者：
DAVIS, BB
中科院分区：
文献类型：
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作者：
BROWN, WW;ZENSER, TV;DAVIS, BB
本文研究了家兔膀胱合成前列腺素E_2（PGE_2）的情况。在[14 C]-花生四烯酸转化为[14 C] PGE 2后，通过TLC分析或通过特定放射免疫测定技术评估PGE 2合成。完整的膀胱和微粒体制备的膀胱移行上皮（粘膜）层和外囊泡层证明合成PGE 2。花生四烯酸增加PGE 2的合成，吲哚美辛阻断。当内部介质（浸浴移行上皮）含有[14 C]花生四烯酸时，在外部介质（浸浴外层）中未观察到可检测的放射性。相反，当外部培养基含有[14 C]花生四烯酸时，在内部培养基中未观察到可检测的放射性。此外，[14 C]花生四烯酸仅掺入直接暴露于含有标记的浸浴介质的组织中。表明兔膀胱具有合成前列腺素E_2的能力，且膀胱移行上皮和膀胱外层合成前列腺素E_2的系统可能不同。
Synthesis of prostaglandin E2 (PGE2) by rabbit bladder was examined. PGE2 synthesis was assessed by TLC analysis after conversion of [14C]-arachidonic acid to [14C]PGE2 or by a specific radioimmunoassay technique. Intact bladder and microsomes prepared from the bladder transitional epithelium (mucosal) layer and the outer vesicular layer demonstrated synthesis of PGE2. PGE2 synthesis was increased by arachidonic acid and blocked by indomethacin. When the inside medium (bathing the transitional epithelium) contained [14C]arachidonic acid, no detectable radioactivity was observed in the outside medium (bathing the outer layer). Conversely, when the outside medium contained [14C]arachidonic acid, no detectable radioactivity was observed in the inside medium. In addition, [14C]arachidonic acid was incorporated only into tissue directly exposed to bathing media containing the label. Evidently the rabbit bladder can synthesize PGE2 and the PGE2-synthesizing systems of the transitional epithelium and outer layer of bladder may be distinct.