Application of magnetic chromatography to the isolation of lysosomes from fibroblasts of patients with lysosomal storage disorders
Application of magnetic chromatography to the isolation of lysosomes from fibroblasts of patients with lysosomal storage disorders
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DOI：
10.1016/s0014-5793(98)01578-6
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发表时间：
1998-12-28
期刊：
影响因子：
3.5
通讯作者：
Winchester, BG
中科院分区：
文献类型：
--
作者：
Diettrich, O;Mills, K;Winchester, BG
已经开发了一种从成纤维细胞中纯化溶酶体的方法，该方法利用超顺磁性胶体右旋糖酐铁颗粒的内吞作用，然后在磁场中分离含铁溶酶体。这使得可以从患有婴儿唾液酸贮积症和其他溶酶体贮积病的患者的成纤维细胞中分离溶酶体，在这些疾病中，由贮藏材料引起的溶酶体密度的变化阻止了通过Percoll梯度离心进行的纯化。根据溶酶体标记酶活性和2D-PAGE判断，从这些细胞中分离的磁性溶酶体与来自正常细胞的磁性溶酶体非常相似。 分析富集的蛋白质。 (C) 1998 年欧洲生化学会联合会。
A method for the purification of lysosomes from fibroblasts has been developed which uses endocytosis of superparamagnetic colloidal iron dextran particles followed by separation of the iron-containing lysosomes in a magnetic field. This permitted isolation of lysosomes from fibroblasts from patients with infantile sialic acid storage disorder and other lysosomal storage diseases in which a shift in lysosomal density induced by the storage material prevents purification by centrifugation in a Percoll gradient, The magnetic lysosomes isolated from these cells are very similar to those from normal cells as judged by lysosomal marker enzyme activity and 2D-PAGE analysis of the enriched proteins. (C) 1998 Federation of European Biochemical Societies.