Diverse biological effects of glycosyltransferase genes from Tartary buckwheat
Diverse biological effects of glycosyltransferase genes from Tartary buckwheat
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苦荞糖基转移酶基因的多种生物学效应
DOI：
10.1186/s12870-019-1955-z
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发表时间：
2019-08
影响因子：
5.3
通讯作者：
Haixia Zhao
中科院分区：
文献类型：
--
作者：
Panfeng Yao;Renyu Deng;Yunji Huang;Simon Stael;Jiaqi Shi;Guanlan Shi;Bingbing Lv;Qi Li;Qixin Dong;Qi Wu;Chenglei Li;Hui Chen;Haixia Zhao
研究背景苦荞（Fagopyrum tataricum）是一种可食用的谷类作物，其芽苗菜因其含有较高水平的抗氧化剂（包括芦丁和花青素）而被市场化和商业化。UDP-葡萄糖类黄酮糖基转移酶（UFGT）在植物类黄酮的生物合成中起着重要作用。到目前为止，有关UFGT基因在苦荞黄酮类化合物生物合成中的作用的研究还很少。在这里，我们报告的鉴定和功能特性的七个UFGT从苦荞麦，可能参与类黄酮的生物合成（并有不同的影响植物生长和发育时，在拟南芥过表达。）结果FtUFGT 6、FtUFGT 7、FtUFGT 8、FtUFGT 9、FtUFGT 15、FtUFGT 40和FtUFGT 41 7个蛋白在拟南芥中可分为3个功能亚群，分别与类黄酮合成和花青素积累有关。冷胁迫后，FtUFGT 8和FtUFGT 15的表达量与苦荞麦花青苷积累能力呈显著正相关。FtUFGT 8、FtUFGT 15和FtUFGT 41在拟南芥中的过量表达显著提高了转基因植株中花色素苷的含量。出乎意料的是，FtUFGT 6的过表达虽然没有导致花青素苷积累增加，但显著提高了转基因植物的生长产量。当野生型植物只有子叶时，大多数FtUFGT 6的转基因植物都长出了真叶。此外，转oxFtUFGT 6基因植株根系的生长速度也明显快于野生型。结论从苦荞麦中分离到7个FtUFGT，其中FtUFGT 8、FtUFGT 15和FtUFGT 41能显著提高转基因植株中总花色苷的积累。此外，FtUFGT 6的过表达增加了转基因植株在所有生长阶段的总产量。而FtUFGT 15在生长后期表现出相反的趋势，延缓了植株的生长速度。这些结果表明，苦荞中FtUFGT基因的生物学功能具有多样性。
BackgroundTartary buckwheat (Fagopyrum tataricum) is an edible cereal crop whose sprouts have been marketed and commercialized for their higher levels of anti-oxidants, including rutin and anthocyanin. UDP-glucose flavonoid glycosyltransferases (UFGTs) play an important role in the biosynthesis of flavonoids in plants. So far, few studies are available on UFGT genes that may play a role in tartary buckwheat flavonoids biosynthesis. Here, we report on the identification and functional characterization of seven UFGTs from tartary buckwheat that are potentially involved in flavonoid biosynthesis (and have varying effects on plant growth and development when overexpressed inArabidopsis thaliana.)ResultsPhylogenetic analysis indicated that the potential function of the seven FtUFGT proteins, FtUFGT6, FtUFGT7, FtUFGT8, FtUFGT9, FtUFGT15, FtUFGT40, and FtUFGT41, could be divided into threeArabidopsis thalianafunctional subgroups that are involved in flavonoid biosynthesis of and anthocyanin accumulation. A significant positive correlation betweenFtUFGT8andFtUFGT15expression and anthocyanin accumulation capacity was observed in the tartary buckwheat seedlings after cold stress. Overexpression inArabidopsis thalianashowed thatFtUFGT8,FtUFGT15, andFtUFGT41significantly increased the anthocyanin content in transgenic plants. Unexpectedly, overexpression ofFtUFGT6,while not leading to enhanced anthocyanin accumulation,significantly enhanced the growth yield of transgenic plants. When wild-type plants have only cotyledons, most of the transgenic plants ofFtUFGT6had grown true leaves. Moreover, the growth speed of theoxFtUFGT6transgenic plant root was also significantly faster than that of the wild type. At later growth,FtUFGT6transgenic plants showed larger leaves, earlier twitching times and more tillers than wild type, whereasFtUFGT15showed opposite results.ConclusionsSevenFtUFGTswere isolated from tartary buckwheat.FtUFGT8,FtUFGT15, andFtUFGT41can significantly increase the accumulation of total anthocyanins in transgenic plants. Furthermore, overexpression ofFtUFGT6increased the overall yield ofArabidopsistransgenic plants at all growth stages. However,FtUFGT15shows the opposite trend at later growth stage and delays the growth speed of plants. These results suggested that the biological function ofFtUFGTgenes in tartary buckwheat is diverse.
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茶（Camellia sinensis）中涩味化合物生物合成所涉及的 UDP-糖基转移酶的鉴定
影响因子：
6.9
作者：
Cui L;Yao S;Dai X;Yin Q;Liu Y;Jiang X;Wu Y;Qian Y;Pang Y;Gao L;Xia T
通讯作者：
Xia T
DOI：
10.1073/pnas.85.15.5536
发表时间：
1988-08-01
影响因子：
11.1
作者：
VALVEKENS, D;VANMONTAGU, M;VANLIJSEBETTENS, M
通讯作者：
VANLIJSEBETTENS, M
影响因子：
5.3
作者：
通讯作者：
--
影响因子：
4.4
作者：
Baba SA;Mohiuddin T;Basu S;Swarnkar MK;Malik AH;Wani ZA;Abbas N;Singh AK;Ashraf N
通讯作者：
Ashraf N
影响因子：
5.6
作者：
Du H;Liu H;Xiong L
通讯作者：
Xiong L