'In-field' and 'out-of-field' functional impairment during subacute and chronic phases of experimental radiation enteropathy in the rat
'In-field' and 'out-of-field' functional impairment during subacute and chronic phases of experimental radiation enteropathy in the rat
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DOI：
10.1080/0955300031000150594
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发表时间：
2003-06-01
影响因子：
2.6
通讯作者：
Griffiths, NM
中科院分区：
文献类型：
--
作者：
François, A;Milliat, F;Griffiths, NM
目的：研究大鼠小肠暴露段和屏蔽段（近端和远端）局部照射的亚急性和慢性功能后果。材料和方法：使用阴囊疝气手术模型。回肠环暴露于单剂量的 18、21 或 29.6 Gy X 射线照射。照射后 2 周和 26 周对上皮结构和转运能力进行跟踪。结果：受照射的节段显示粘膜溃疡，随后出现透壁纤维化。运输能力受损 2 至 26 周。在近端节段发现亚急性功能障碍，没有形态改变或中性粒细胞流入。 26 周时，近端和远端节段均显示出上皮转运能力受损，在 21 Gy 照射的情况下，中性粒细胞流入粘膜下层，在 29.6 Gy 照射后，中性粒细胞流入粘膜下层和固有肌层。 结论：放射性肠炎的特点是照射野内外的功能损害。在亚急性期，受照射的节段可能是介质的来源，可能通过血流和/或肠神经系统影响损伤部位外的肠道功能。慢性期肠闭塞综合征的发生可能是导致肠道功能障碍的原因，但并不排除小肠受保护部分可能发生炎症过程。
Purpose: To investigate subacute and chronic functional consequences of localized irradiation of rat small intestine on exposed and shielded segments ( proximal and distal).Materials and methods: The surgical model of a scrotal hernia was used. The ileal loop was exposed to single doses of 18, 21 or 29.6 Gy X-irradiation. Epithelial structure and transport capacity were followed 2 and 26 weeks post-exposure.Results: Irradiated segments showed mucosal ulceration followed by transmural fibrosis. Transport capacity was impaired from 2 to 26 weeks. Subacute functional impairment was noticed in the proximal segment, without either morphological alteration or neutrophil influx. At 26 weeks, both proximal and distal segments showed impaired epithelial transport capacity, with neutrophil influx in the submucosa in cases of 21-Gy exposure and in the submucosa and muscularis propria after 29.6 Gy.Conclusions: Radiation enteritis was characterized by functional impairment, within as well as outside, the irradiation field. During the subacute phase, the irradiated segment may be a source of mediators which might influence intestinal function outside the site of injury via the blood stream and/or enteric nervous system. The development of an intestinal occlusion syndrome during the chronic phase might be responsible for intestinal dysfunction but it does not rule out a possible inflammatory process developing in the shielded parts of the small intestine.