Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1 (AtBl-1)
Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1 (AtBl-1)
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DOI：
10.1073/pnas.211423998
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发表时间：
2001-10-09
影响因子：
11.1
通讯作者：
Uchimiya, H
中科院分区：
文献类型：
--
作者：
Kawai-Yamada, M;Jin, LH;Uchimiya, H
我们最近分离出AtBI-1（拟南芥Bax抑制剂-1）基因，其表达抑制了酵母中的细胞死亡。为了确定在植物系统中是否也是如此，产生了在地塞米松（DEX）诱导型启动子下过表达Bax蛋白的转基因拟南芥植物。在DEX处理时，这样的转基因植物在整个植物水平上表现出显著的细胞死亡、细胞皱缩、膜破坏和其他凋亡表型。用含有在花椰菜花叶病毒35 S启动子控制下的AtBI-1基因（用绿色荧光蛋白标记）的载体再转化转基因Bax植物。表达Bax和AtBI-1的植物能够通过维持细胞内完整性来维持DEX处理的生长。因此，我们在这里提出的直接遗传证据表明，植物抗凋亡蛋白AtBI-1是生物活性抑制哺乳动物Bax的行动在植物。
We recently isolated the AtBI-1 (Arabidopsis Bax Inhibitor-1) gene, the expression of which suppressed Bax-induced cell death in yeast. To determine whether the same is true in the plant system, transgenic Arabidopsis plants overexpressing Bax protein under a dexamethasone (DEX)-inducible promoter were generated. On DEX treatment, such transgenic plants exhibited marked cell death at the whole-plant level, cell shrinkage, membranous destruction, and other apoptotic phenotypes. Transgenic Bax plants were retransformed with a vector containing the AtBI-1 gene (tagged with green fluorescent protein) under the control of the cauliflower mosaic virus 35S promoter. Plants expressing both Bax and AtBI-1 were able to maintain growth on DEX-treatment by sustaining intracellular integrity. Thus, we present here direct genetic evidence that the plant antiapoptotic protein AtBI-1 is biologically active in suppressing the mammalian Bax action in planta.