MECHANISM OF PEPTIDE BOND FORMATION IN POLYPEPTIDE SYNTHESIS
MECHANISM OF PEPTIDE BOND FORMATION IN POLYPEPTIDE SYNTHESIS
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DOI：
10.1073/pnas.51.6.1291
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发表时间：
1964-01-01
影响因子：
11.1
通讯作者：
SCHWEET, R
中科院分区：
文献类型：
--
作者：
ARLINGHAUS, R;SHAEFFER, J;SCHWEET, R
The formation of the active amino acyl-RNA-messenger RNA-ribosome complex is an enzymatic reaction. The "binding" enzyme and GTP are required. The movement of the messenger RNA in relation to the ribosome requires peptide bond synthesis. Since uncharged transfer RNA does not participate in the binding reaction, after a peptide bond is formed, presumably the uncharged RNA leaves the ribosome by diffusion rather than by an exchange mechanism. This mechanism differs considerably from that proposed for E. coli. The major experimental differences are the nonenzymatic nature of the binding and the participation of uncharged transfer RNA. In the E. coli system, Nakamoto et al. have also reported the nonenzymatic binding of phe-RNA to ribosomes in the presence of poly U.
