IMMUNITY TO MYCOPLASMA-BOVIS INFECTIONS OF THE RESPIRATORY-TRACT OF CALVES
IMMUNITY TO MYCOPLASMA-BOVIS INFECTIONS OF THE RESPIRATORY-TRACT OF CALVES
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DOI：
10.1016/s0034-5288(18)32755-3
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发表时间：
1980-01-01
影响因子：
2.4
通讯作者：
TAYLOR, G
中科院分区：
文献类型：
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作者：
HOWARD, CJ;GOURLAY, RN;TAYLOR, G
小牛肌内接种福尔马林灭活的牛分枝杆菌加佐剂，14天后气管内注射灭活的微生物，不加佐剂。 7 天后，对它们进行气管内攻击，攻击后 21 天，它们肺部的牛分枝杆菌明显少于对照小牛。即时两点或 2 次气管内接种灭活的牛支原体不会诱导耐药性，但 3 次皮下注射可刺激免疫力。用佐剂接种灭活支原体。免疫与通过单径向溶血技术可在肺洗液中检测到的抗体[Ab]相关。综合即时通讯气管内给予灭活的牛分枝杆菌，在肺洗液中诱导出比 2 肌内更好的抗体反应。或 2 次气管内接种。在皮下接种 3 次的小牛的肺洗液中检测到抗体。但它显然是从血清中提取的。通过放射免疫测定，通过单次放射状溶血在肺洗液中检测到的抗体是Ig[免疫球蛋白]G。
Calves were inoculated i.m. with formalin-killed M. bovis with adjuvant and after 14 days intratracheally with killed organisms without adjuvant. Seven days later they were challenged intratracheally and 21 days after challenge they had significantly fewer M. bovis in their lungs than control calves. Two i.m. or 2 intratracheal inoculations of killed M. bovis did not induce resistance but immunity could be stimulated by 3 s.c. inoculations of inactivated mycoplasmas with adjuvant. Immunity was correlated with antibody [Ab] detectable in lung washings by the single radial hemolysis technique. Combined i.m. and intratracheal administration of killed M. bovis induced a better Ab response in lung washings than 2 i.m. or 2 intratracheal inoculations. Ab was detected in lung washings from calves inoculated 3 times s.c. but it was apparently derived from serum. The Ab detected in lung washings by single radial hemolysis was Ig[immunoglobulin]G by radioimmunoassay.