The proliferation and differentiation of the bone-lining cell in estrogen-induced osteogenesis.
The proliferation and differentiation of the bone-lining cell in estrogen-induced osteogenesis.
复制标题
雌激素诱导的成骨过程中骨衬细胞的增殖和分化。
DOI：
10.1016/8756-3282(86)90255-3
复制
发表时间：
1986
期刊：
影响因子：
4.1
通讯作者：
Miller,SC
中科院分区：
文献类型：
--
作者：
Bowman,BM;Miller,SC
本研究的目的是确定骨衬细胞的能力，其中包括大多数非重塑骨表面的成年骨骼，增殖和促进快速骨内膜成骨反应后，雌激素治疗雄性日本鹌鹑。在对照组中，未处理的鸟骨衬细胞覆盖大部分股骨骨内膜表面。骨衬细胞沿着骨表面稀疏延伸，具有扁平的细胞核，约占骨表面附近细胞的77%。对照组骨表面细胞的线密度约为21个细胞/mm骨表面。雌激素给药后16-20 h，在骨表面可见较大的圆形细胞核，其中一些细胞含有~ 3 H-胸腺嘧啶核苷（~ 3 H-TdR）标记。在雌激素给药后的第一个24小时内，线性骨表面细胞密度增加到约38个细胞/mm。在雌激素给药后20-30小时，大多数邻近骨表面的细胞是圆形的。雌激素给药后36 h，骨表面细胞的~ 3 H-TdR标记出现峰值。48 h时，骨表面已被成骨细胞覆盖，并有髓骨形成。这些结果表明，在成人骨衬里细胞似乎保留了一些增殖能力和成骨潜力。然而，由于细胞密度的快速增加早于3 H-TdR标记的快速增加，以及骨表面出现有丝分裂像，另一种细胞来源也可能有助于雌激素诱导的成骨反应。
The purpose of this study was to determine the capacity of the bone-lining cell, which covers most nonremodeling bone surfaces in the adult skeleton, to proliferate and contribute to the rapid endosteal osteogenic response following estrogen treatment in male Japanese quail. In control, untreated birds bone-lining cells cover most of the femoral endosteal surface. Bone-lining cells are thinly extended along bone surfaces, have flat nuclei, and account for about 77% of the cells adjacent to the bone surface. The lineal density of bone surface cells in the controls was about 21 cells/mm of bone surface. By 16–20 h after estrogen administration cells with larger, round nuclei were seen on the bone surface and some of these cells contained a3H-thymidine (3H-TdR) label. Within the first 24 h after estrogen administration the lineal bone surface cell density had increased to about 38 cells/mm. At 20–30 h after estrogen administration, most of the cells adjacent to the bone surface were round. The peak3H-TdR labeling of cells immediately adjacent to the bone surface occurred about 36 h after estrogen administration. By 48 h the bone surface was covered with osteogenic cells and developing medullary bone. These results suggest that the bone-lining cell in the adult appears to retain some proliferative capability and osteogenic potential. However, because a rapid increase in cell density began prior to the rapid increases in3H-TdR labeling, as well as the appearance of mitotic figures on the bone surface, another source of cells may have also contributed to the osteogenic response induced by estrogen.