Spironucleus vortens, a possible cause of hole-in-the-head disease in cichlids.
Spironucleus vortens, a possible cause of hole-in-the-head disease in cichlids.
复制标题
漩涡螺核，可能是慈鲷头部洞病的原因。
DOI：
10.3354/dao045197
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发表时间：
2001
影响因子：
1.4
通讯作者：
R. Matthews
中科院分区：
文献类型：
--
作者：
GC Paull;R. Matthews
在英国西南部的当地水族馆获得的11只盘鱼Symphysodon discus Heckel，1840和1只神仙鱼Pterophyllum scalare Lichelus，1823中记录了头部穿孔疾病。涡状螺旋核菌波因顿等人，1995年，从显示严重疾病体征的盘鱼的肾、肝、脾和头部病变中以及从所有鱼的肠中分离。在神仙鱼的头部病变中也记录到了六肽组。经鉴定，这些鞭毛虫为S.基于借助于SEM观察到的形貌特征而涡旋。本研究成功地建立了一种改良的体外培养方法，用于沙门氏菌的检测、分离和长期维持。漩涡。以3至5 d的间隔对鞭毛虫进行传代培养，新培养基补充有来自无感染的尼罗罗非鱼（Oreochromis niloticus，Linnaeus，1757）的新鲜肝脏。并结合S.在通过消化道的全身性感染后，作为头部穿孔疾病的病原体而发生。
Hole-in-the-head disease is recorded in 11 discus Symphysodon discus Heckel, 1840 and 1 angelfish Pterophyllum scalare Lichtenstein, 1823 obtained from local aquarists within the Southwest of the UK. Spironucleus vortens Poynton et al. 1995, was isolated from the kidney, liver, spleen and head lesions of discus showing severe signs of the disease and from the intestines of all fish. The hexamitid was also recorded from the head lesions of the angelfish. The identity of these flagellates was confirmed as S. vortens on the basis of topographical features seen with the aid of SEM. A modified in vitro culture method was successfully developed for the detection, isolation and long-term maintenance of S. vortens. The flagellate was sub-cultured at 3 to 5 d intervals, new media being supplemented with fresh liver from Oreochromis niloticus (Linnaeus, 1757) free from infection. The results are discussed in relation to S. vortens as the causative agent for hole-in-the-head disease following systemic infection via the digestive tract.