In vitro assessment of encapsulated C3A hepatocytes functions in a fluidized bed bioreactor
In vitro assessment of encapsulated C3A hepatocytes functions in a fluidized bed bioreactor
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DOI：
10.1021/bp034301z
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发表时间：
2004-07-01
影响因子：
2.9
通讯作者：
Legallais, C
中科院分区：
文献类型：
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作者：
David, B;Dufresne, M;Legallais, C
在本体外模型中，作者旨在评估包封在藻酸盐珠中并在生物反应器中进行流化床运动的肝细胞的活力和功能。选择人永生化C3A细胞系作为细胞模型。实施两种对照，其由（1）在烧瓶上培养的细胞和（2）在静态条件下包封在藻酸盐珠中的细胞组成。研究的细胞功能包括总蛋白、白蛋白、尿素和氨合成，以及过量情况下的氨清除。三种情况的比较表明，海藻酸盐的三维结构为细胞功能提供了一个合适的环境。此外，与静态情况相比，流化床生物反应器增强了传质，从而增加了从珠粒中释放出的物质的量。氨解毒作用仅因封装而降低。该体外模型证实了流化床生物人工肝的概念，表明其可以有效地保留细胞功能。此外，就尿素和蛋白质的合成和释放而言，MA细胞系的使用，结合包封和流化技术，为体外肝脏供应的目的提供了真实的潜力。
In the present in vitro model, the authors intended to assess viability and functionality of hepatocytes encapsulated into alginate beads and submitted to a fluidized bed motion in a bioreactor. Human immortalized C3A line was chosen as cell model. Two controls consisting of (1) cells cultured on flasks and (2) cells encapsulated in alginate beads under static conditions were implemented. The cell functions studied were total protein, albumin, urea, and ammonia synthesis, as well as ammonia removal in the case of overdose. The comparison among the three cases studied showed that the three-dimensional structure of alginate offered a suitable environment for cell functions. In addition, the fluidized bed bioreactor enhanced the mass transfer and thus increased the amount of species released out of the beads, as compared with the static case. Ammonia detoxification only appeared reduced by encapsulation. The concept of a fluidized bed bioartificial liver was thus validated by this in vitro model, which indicated that cell functions could be efficiently retained. In addition, as far as urea and protein synthesis and release were concerned, the use of the MA cell line, in combination with encapsulation and fluidization technology, offered a real potentiality for the purpose of extracorporeal liver supply.