TIME SERIES ANALYSIS OF IMPULSE SEQUENCES OF THALAMIC SOMATIC SENSORY NEURONS.
TIME SERIES ANALYSIS OF IMPULSE SEQUENCES OF THALAMIC SOMATIC SENSORY NEURONS.
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丘脑躯体感觉神经元脉冲序列的时间序列分析。
DOI：
10.1152/jn.1964.27.4.517
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发表时间：
1964
影响因子：
2.5
通讯作者：
L. Viernstein
中科院分区：
文献类型：
--
作者：
G. Poggio;L. Viernstein
方法对35只未麻醉的猕猴进行实验。在最终实验前约1个月对每只动物进行头部传入阻滞。动物的手术过程、术后状况和护理已在其他地方描述过(14)。在实验过程中，用三硫碘化镓将动物维持在神经肌肉麻痹状态并进行人工通气。所有产生疼痛的手术都仅限于传入神经迟钝的头部。许多实验都监测了呼气末 CO、血压和脑电图。观察到动物的状态在警觉性和浅睡眠之间摇摆。对背侧丘脑腹基底复合体、外部的单个丘系神经元的脉冲活动的一系列记录进行了分析。通过尖端直径为 3-4 p 的铟填充微电极从细胞外位置记录活性。用于记录和处理数据的仪器和技术已在先前的出版物中描述过(14)，以及用于识别丘脑内电极位置的解剖方法。研究了与皮肤或深层外周受体相关的丘月层神经元。对于定义为“关节神经元”的丘脑神经元，即对肢体的旋转和位置做出反应的细胞，通过允许预设关节转动角度在 2 度以内、速度在 1 度/组以内的仪器来传递受控外周刺激。(13)。对皮肤和筋膜受体的刺激没有得到很好的控制，并且通常是最大的，通过将杆或刷子稳定地施加到所研究的神经元的外周感受野的中心来完成。对自发活动和驱动活动的记录进行了分析。术语“自发”用于描述在没有有意和特定的感觉刺激的情况下进行的活动。显然，该术语指的是神经放电序列，可能取决于影响所研究的神经元的各种机制，并且已在其他地方概述（18）。仅当由特定外周受体的刺激建立的传入脉冲在中继后撞击时，才会产生丘系神经元的不同且通常可识别的活动状态，即驱动状态。所研究的细胞所属的神经池在自发活动期间运行的机制与驱动活动期间运行的机制在何种程度上和以何种形式相互作用，目前还无法确定。
METHODSExperiments were conducted on 35 unanesthetized macaque monkeys. In each animal a deafferentation of the head was performed about 1 month before the terminal experiment. The surgical procedure and postoperative condition and care of the animals have been described elsewhere(14). During the experiment the animal was maintained under neuromuscular paralysis with gallamine triethiodide and artificially ventilated. All pain-producing procedures were confined to the deafferented head. End-tidal COs, blood pressure, and the electroencephalogram were monitored in many experiments. The state of the animal was observed to oscillate between alertness and light sleep. The analyses were performed on a series of records of the impulse activity of single lemniscal neurons in the ventrobasal complex, pars externa, of the dorsal thalamus. The activity was recorded from an extracellular position by means of indium-filled microelectrodes, with tip diameters of 3-4 p. Instruments and techniques used for the recording and processing of data have been described in a previous publication(14), together with the anatomical methods employed for the identification of electrode positions within the thalamus. Lemniscal neurons related to the skin or deep peripheral receptors were studied. For the thalamic neurons defined as “joint neurons,” ie, cells which respond to rotation and position of a limb, controlled peripheral stimulation was delivered by an instrument which permitted presetting the angle of the joint turned to within 2 deg., and the velocity to within 1 deg./set.(13). Stimulation of the skin and of fascial receptors was not well controlled and it was usually maximal, done with rods or brushes steadily applied to the center of the peripheral receptive field for the neuron under study. Analyses were made on records of spontaneous and driven activity. The term% pontaneous” is used to describe that activity which goes on in the absence of an intentional and specific sensory stimulus. Obviously this term refers to neural discharge sequences which may depend on a variety of mechanisms influencing the neuron under study, and which have been outlined elsewhere (18). A different and usually recognizable state of activity of the lemniscal neuron, the driven state, is brought about only when afferent impulses, set up by the stimulation of the specific peripheral receptors, impinge after relay upon the neural pool of which the cell under study is a member. To what extent and in which form the mechanisms operating during spontaneous activity interplay with those operating during the driven one it is not possible to determine at the present time.