Structure of a sugar chain of a protease inhibitor isolated from barbados pride (Caesalpinia pulcherrima Sw.) seeds.
Structure of a sugar chain of a protease inhibitor isolated from barbados pride (Caesalpinia pulcherrima Sw.) seeds.
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从巴巴多斯骄傲（Caesalpinia pulcherrima Sw.）种子中分离出的蛋白酶抑制剂的糖链结构。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
T. Ikenaka
中科院分区：
文献类型：
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作者：
S. Hase;S. Koyama;H. Daiyasu;H. Takemoto;S. Hara;Y. Kobayashi;Y. Kyōgoku;T. Ikenaka
一个天冬酰胺连接的糖链的蛋白酶抑制剂从马蹄莲（Caesalpinia pulcherrima Sw.）通过肼解作用释放。N-乙酰化后，该碳水化合物单元的还原末端残基与2-氨基吡啶偶联，并通过凝胶过滤和反相HPLC纯化吡啶基氨基（PA-）衍生物。所得PA-糖链的结构主要通过逐步外切糖苷酶二聚体和500 MHz 1H-NMR光谱测定，并证明如下：（式;见正文）。
An asparagine-linked sugar chain of a protease inhibitor from barbados pride (Caesalpinia pulcherrima Sw.) was liberated by hydrazinolysis. After N-acetylation, the reducing end residue of this carbohydrate unit was coupled with 2-aminopyridine and the pyridylamino (PA-) derivative was purified by gel-filtration and reversed-phase HPLC. The structure of the resulting PA-sugar chain was determined mainly by stepwise exoglycosidase digestions and 500 MHz 1H-NMR spectroscopy and proved to be as follows: (formula; see text).